The t complex-encoded GTPase-activating protein Tagap1 acts as a transmission ratio distorter in mice
The t complex-encoded GTPase-activating protein Tagap1 acts as a transmission ratio distorter in mice
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DOI：
10.1038/ng1617
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发表时间：
2005-09-01
期刊：
影响因子：
30.8
通讯作者：
Herrmann, BG
中科院分区：
文献类型：
--
作者：
Bauer, H;Willert, JR;Herrmann, BG
鼠标传动比失真是由几个t复失真器（Tcds）反式作用于t复响应器（Tcr）引起的（1 - 4）。Tcds累加地影响来自t/+雄性的所有精子的鞭毛运动；携带Tcr的精子被拯救，从而使t精子在受精中占有优势。在这里，我们展示了Tagap1，一个GTPase激活蛋白，可以作为一个扭曲。Tagap1映射到Tcd1区间，有4个t位点，编码改变的蛋白质，包括C末端截断的形式。野生型Tagap1在精子细胞中的过度表达使Tcd功能表型化，而功能缺失的Tagap1等位基因降低了t(6)单倍型的传输率。综合数据强烈提示，Tcd1a是由Tagap1的t个位点产生的。我们的研究结果揭示了Tcd的分子性质，并证明了小G蛋白在小鼠传输比畸变中的重要性。
Transmission ratio distortion in the mouse is caused by several t- complex distorters ( Tcds) acting in trans on the t- complex responder ( Tcr)(1 - 4). Tcds additively affect the flagellar movement of all spermatozoa derived from t/+ males; sperm carrying Tcr are rescued, resulting in an advantage for t sperm in fertilization. Here we show that Tagap1, a GTPase- activating protein, can act as a distorter. Tagap1 maps to the Tcd1 interval and has four t loci, which encode altered proteins including a C- terminally truncated form. Overexpression of wild- type Tagap1 in sperm cells phenocopied Tcd function, whereas a loss- of- function Tagap1 allele reduced the transmission rate of the t(6) haplotype. The combined data strongly suggest that the t loci of Tagap1 produce Tcd1a. Our results unravel the molecular nature of a Tcd and demonstrate the importance of small G proteins in transmission ratio distortion in the mouse.