Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media
Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media
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DOI：
10.1111/j.1567-1364.2009.00533.x
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发表时间：
2009-11-01
影响因子：
3.2
通讯作者：
Sullivan, Derek J.
中科院分区：
文献类型：
--
作者：
Citiulo, Francesco;Moran, Gary P.;Sullivan, Derek J.
白色念珠菌和都柏林念珠菌是唯一被观察到产生厚垣孢子的念珠菌属。这些大型厚壁细胞的功能目前尚不清楚。在这份报告中，我们描述了生产和纯化的厚垣孢子从这些物种在确定的液体培养基。用荧光染料FUN-1染色表明厚垣孢子是代谢活性细胞，但在> 30天龄的厚垣孢子中检测不到代谢活性。然而，5-15日龄的厚垣孢子可以被诱导产生子厚垣孢子、芽生孢子、假菌丝和真菌丝，这取决于所使用的培养条件。还观察到预先诱导萌发的厚垣孢子在吞噬作用后从鼠巨噬细胞中逃逸，这表明这些结构在体内可能是可行的。菌丝附着和纯化的厚垣孢子在水中和干燥胁迫下迅速失去活力，这表明它们不太可能作为长期储存结构。相反，我们的数据表明厚垣孢子代表了C.白色念珠菌和C.都柏林的。
Candida albicans and Candida dubliniensis are the only Candida sp. that have been observed to produce chlamydospores. The function of these large, thick-walled cells is currently unknown. In this report, we describe the production and purification of chlamydospores from these species in defined liquid media. Staining with the fluorescent dye FUN-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are > 30 days old. However, 5-15-day-old chlamydospores could be induced to produce daughter chlamydospores, blastospores, pseudohyphae and true hyphae depending on the incubation conditions used. Chlamydospores that were preinduced to germinate were also observed to escape from murine macrophages following phagocytosis, suggesting that these structures may be viable in vivo. Mycelium-attached and purified chlamydospores rapidly lost their viability in water and when subjected to dry stress, suggesting that they are unlikely to act as long-term storage structures. Instead, our data suggest that chlamydospores represent an alternative specialized form of growth by C. albicans and C. dubliniensis.