Imprinting of an evolutionarily conserved antisense transcript gene APeg3
Imprinting of an evolutionarily conserved antisense transcript gene APeg3
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DOI：
10.1016/j.gene.2007.10.036
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发表时间：
2008-02-15
期刊：
影响因子：
3.5
通讯作者：
Kim, Joomyeong
中科院分区：
文献类型：
--
作者：
Choo, Jung Ha;Kim, Jeong Do;Kim, Joomyeong
APeg 3是最近从大鼠脑组织中发现的Peg 3的反义转录基因。EST数据库的仔细分析表明，同源转录本也存在于其他哺乳动物物种，包括小鼠，牛和人类。5 '-和3'-RACE实验随后从小鼠脑中鉴定了APeg 3的900-bp cDNA序列。小鼠APeg 3位于Peg 3的3 'UTR，具有无内含子基因组结构。小鼠APeg 3的表达主要来源于父系等位基因，表明该反义转录基因在脑中存在印迹。链特异性RNA分析还揭示了人和牛APEG 3在成年大脑中的表达。总之，我们的研究证实，哺乳动物PEG 3位点窝藏一个反义转录基因显示父系等位基因特异性表达，进化保守性进一步表明该转录基因的功能，这个印记域的潜在作用。由爱思唯尔公司出版
APeg3 is an antisense transcript gene of Peg3, which has been recently identified from rat brain. Careful analyses of EST databases indicated that a homologous transcript also exists in other mammalian species, including mouse, cow and human. 5'-and 3'-RACE experiments have subsequently identified a 900-bp cDNA sequence of APeg3 from mouse brain. Mouse APeg3 is localized in the 3'UTR of Peg3 with an intronless genomic structure. The expression of mouse APeg3 is derived mainly from the paternal allele, indicating the imprinting of this antisense transcript gene in brain. Strand-specific RNA analyses also revealed the expression of both human and cow APEG3 in adult brains. In sum, our study confirms that the mammalian PEG3 locus harbors an antisense transcript gene displaying paternal allele-specific expression, and the evolutionary conservation further suggests potential roles of this transcript gene for the function of this imprinted domain. Published by Elsevier B.V.