Surface-assisted laser desorption/ionization mass spectrometry imaging: A review.
Surface-assisted laser desorption/ionization mass spectrometry imaging: A review.
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DOI：
10.1002/mas.21670
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发表时间：
2022-05
影响因子：
6.6
通讯作者：
中科院分区：
文献类型：
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在过去的几十年里，表面辅助激光解吸/电离质谱学(SALDI-MS)因其独特的能力而引起人们越来越多的兴趣，这种能力可以通过用于促进分析物解吸/电离的纳米结构底物来实现。虽然Saldi-MS最广为人知的资产是对小分子的非靶向分析，但这项技术也提供了靶向方法的可能性。特别是，SALDI-MS成像(SALDI-MSI)的实施开辟了新的机会，这是本次审查的重点。在简要讨论了与这项技术相关的命名和基本机制之后，我们广泛讨论了实施Saldi-MSI的分析策略。重点不仅在于样品的制备，还在于纳米底物的选择(在化学成分和形态方面)及其功能化的可能性，以便以有针对性的方法对特定化合物进行选择性分析。随后，介绍了Saldi-MSI在不同领域(即生物医学、生物、环境和法医学)中的一些精选应用。结论部分总结了Saldi-MSI的优点和尚存的局限性，并对该技术的发展前景进行了展望。
In the last decades, surface‐assisted laser desorption/ionization mass spectrometry (SALDI‐MS) has attracted increasing interest due to its unique capabilities, achievable through the nanostructured substrates used to promote the analyte desorption/ionization. While the most widely recognized asset of SALDI‐MS is the untargeted analysis of small molecules, this technique also offers the possibility of targeted approaches. In particular, the implementation of SALDI‐MS imaging (SALDI‐MSI), which is the focus of this review, opens up new opportunities. After a brief discussion of the nomenclature and the fundamental mechanisms associated with this technique, which are still highly controversial, the analytical strategies to perform SALDI‐MSI are extensively discussed. Emphasis is placed on the sample preparation but also on the selection of the nanosubstrate (in terms of chemical composition and morphology) as well as its functionalization possibilities for the selective analysis of specific compounds in targeted approaches. Subsequently, some selected applications of SALDI‐MSI in various fields (i.e., biomedical, biological, environmental, and forensic) are presented. The strengths and the remaining limitations of SALDI‐MSI are finally summarized in the conclusion and some perspectives of this technique, which has a bright future, are proposed in this section.