Sensitive arginine sensing based on inner filter effect of Au nanoparticles on the fluorescence of CdTe quantum dots
Sensitive arginine sensing based on inner filter effect of Au nanoparticles on the fluorescence of CdTe quantum dots
复制标题
基于 Au 纳米颗粒对 CdTe 量子点荧光的内滤效应的灵敏精氨酸传感。
DOI：
10.1016/j.saa.2016.08.057
复制
发表时间：
2017-02-15
影响因子：
4.4
通讯作者：
Ren, Xueqin
中科院分区：
文献类型：
--
作者：
Liu, Haijian;Li, Ming;Ren, Xueqin
精氨酸在许多生物学功能中发挥着重要作用，其检测意义重大。基于柠檬酸盐稳定的金纳米粒子（AuNPs）对巯基乙酸修饰的CdTe量子点（QDs）荧光的内过滤效应（IFE），建立了一种灵敏、简便、经济的精氨酸荧光检测方法。当柠檬酸盐稳定的金纳米粒子与巯基乙酸封端的CdTe量子点混合时，CdTe量子点的荧光通过IFE被金纳米粒子显著猝灭。在精氨酸存在下，精氨酸可诱导金纳米粒子的聚集并改变其吸收光谱，随着精氨酸含量的增加，金纳米粒子的荧光强度逐渐恢复，实现了精氨酸的荧光“开启”传感。阐明了检测机理，并对各种实验条件进行了优化。在最佳条件下，线性范围为16 ~ 121 μ g L ~（-1），检出限为5.614 μ g L ~（-1）。以精氨酸注射液、复方氨基酸注射液、甚至血浆为样品，对精氨酸的分析均取得了满意的结果。因此，该方法具有操作简单、成本低、灵敏度高、选择性好等优点，有望用于生物样品中精氨酸的检测。(C)2016爱思唯尔B. V.保留所有权利。
Arginine plays an important role in many biological functions, whose detection is very significant. Herein, a sensitive, simple and cost-effective fluorescent method for the detection of arginine has been developed based on the inner filter effect (IFE) of citrate-stabilized gold nanoparticles (AuNPs) on the fluorescence of thioglycolic add-capped CdTe quantum dots (QDs). When citrate-stabilized AuNPs were mixed with thioglycolic acid-capped CdTe QDs, the fluorescence of CdTe QDs was significantly quenched by AuNPs via the IFE. With the presence of arginine, arginine could induce the aggregation and corresponding absorption spectra change of AuNPs, which then IFE-decreased fluorescence could gradually recover with increasing amounts of arginine, achieving fluorescence "turn on" sensing for arginine. The detection mechanism is clearly illustrated and various experimental conditions were also optimized. Under the optimum conditions, a decent linear relationship was obtained in the range from 16 to 121 mu g L-1 and the limit of detection was 5.614 mu g L-1. And satisfactory results were achieved in arginine analysis using arginine injection, compound amino acid injection, even blood plasma as samples. Therefore, the present assay showed various merits, such as simplicity, low cost, high sensitivity and selectivity, making it promising for sensing arginine in biological samples. (C) 2016 Elsevier B.V. All rights reserved.