Na+/Cl- cotransporter 2 is not fish-specific and is widely found in amphibians, non-avian reptiles, and select mammals.
Na+/Cl- cotransporter 2 is not fish-specific and is widely found in amphibians, non-avian reptiles, and select mammals.
复制标题
Na /Cl-协同转运蛋白 2 不是鱼类特有的，广泛存在于两栖动物、非鸟类爬行动物和特定哺乳动物中。
DOI：
10.1152/physiolgenomics.00143.2022
复制
发表时间：
2023
影响因子：
4.6
通讯作者：
Kato,Akira
中科院分区：
文献类型：
--
作者：
Motoshima,Toya;Nagashima,Ayumi;Ota,Chihiro;Oka,Haruka;Hosono,Kohei;Braasch,Ingo;Nishihara,Hidenori;Kato,Akira
溶质载流子12 （Slc12）是一类电中性阳离子偶联氯离子（Cl−）共转运体。Na+/K+/2Cl - (Nkcc)和Na+/Cl -共转运体（Ncc）属于Nkcc/Ncc亚家族。人类和小鼠拥有一个Na+/Cl−共转运体基因（nccgene:slc12a3），而硬骨鱼除了它们的物种特异性相似物外，还拥有多个基因，slc12a3（ncc1）和slc12a10（ncc2）。两栖动物和有鳞动物有两个基因：slc12a3（ncc1）和ncc3。然而，slc12a10和ncc3之间的进化关系仍未得到解决，slc12a10（ncc2）在哺乳动物中的存在也未得到澄清。脊椎动物基因组数据库的同源性和系统发育分析表明，ncc3是slc12a10的同源基因，slc12a10存在于大多数鳍状鱼类、腔棘鱼、两栖动物、爬行动物和少数哺乳动物（如鸭嘴兽和马）中，但在鸟类、大多数哺乳动物和部分鳍状鱼类（河豚）中被假源化或缺失。这表明slc12a10广泛存在于骨脊椎动物中，并在多个谱系中独立地假源化或缺失。值得注意的是，与一些显示不同slc12a10组织表达谱的鱼类相比，斑点gar，非洲爪蛙，红耳滑龟和马在卵巢或早熟性腺中表达c12a10。在马的组织中，Slc12a10的剪接变异体被克隆出来，数量之多出乎意料，其中许多变异体编码Slc12a10的截断形式，这表明马蹄铁lc12a10的功能限制被削弱，可能正处于成为假基因的过程中。我们的研究结果阐述了Slc12的Nkcc/Ncc亚家族在脊椎动物中的进化。yslc12a10不是鱼类特有的基因，存在于少数哺乳动物（如鸭嘴兽和马）、非鸟类爬行动物、两栖动物中，但在大多数哺乳动物（如人类、小鼠、猫、牛和犀牛）、鸟类和一些鳍状鱼类（河豚）中被假基因化或删除。
Solute carrier 12 (Slc12) is a family of electroneutral cation-coupled chloride (Cl−) cotransporters. Na+/K+/2Cl−(Nkcc) and Na+/Cl−cotransporters (Ncc) belong to the Nkcc/Ncc subfamily. Human and mouse possess one gene for the Na+/Cl−cotransporter (nccgene:slc12a3), whereas teleost fishes possess multiplenccgenes,slc12a3(ncc1) andslc12a10(ncc2), in addition to their species-specific paralogs. Amphibians and squamates have twonccgenes:slc12a3(ncc1) andncc3. However, the evolutionary relationship betweenslc12a10andncc3remains unresolved, and the presence ofslc12a10(ncc2) in mammals has not been clarified. Synteny and phylogenetic analyses of vertebrate genome databases showed thatncc3is the ortholog ofslc12a10, andslc12a10is present in most ray-finned fishes, coelacanths, amphibians, reptiles, and a few mammals (e.g., platypus and horse) but pseudogenized or deleted in birds, most mammals, and some ray-finned fishes (pufferfishes). This shows thatslc12a10is widely present among bony vertebrates and pseudogenized or deleted independently in multiple lineages. Notably, as compared with some fish that show variedslc12a10tissue expression profile, spotted gar, African clawed frog, red-eared slider turtle, and horse expressslc12a10in the ovaries or premature gonads. In horse tissues, an unexpectedly large number of splicing variants for Slc12a10 have been cloned, many of which encode truncated forms of Slc12a10, suggesting that the functional constraints of horseslc12a10are weakened, which may be in the process of becoming a pseudogene. Our results elaborate on the evolution of Nkcc/Ncc subfamily of Slc12 in vertebrates.NEW & NOTEWORTHYslc12a10is not a fish-specific gene and is present in a few mammals (e.g., platypus and horse), non-avian reptiles, amphibians, but was pseudogenized or deleted in most mammals (e.g., human, mouse, cat, cow, and rhinoceros), birds, and some ray-finned fishes (pufferfishes).