Exopolysaccharides from Lactobacillus kiferi as adjuvant enhanced the immuno-protective against Staphylococcus aureus infection
Exopolysaccharides from Lactobacillus kiferi as adjuvant enhanced the immuno-protective against Staphylococcus aureus infection
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基氏乳杆菌胞外多糖作为佐剂增强对金黄色葡萄球菌感染的免疫保护作用
DOI：
10.1016/j.ijbiomac.2020.06.005
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发表时间：
2020
影响因子：
8.2
通讯作者：
Xiao Wang
中科院分区：
文献类型：
--
作者：
Lei Xiu;Shouxin Sheng;Zhongpeng Hu;Yang Liu;Jianwei Li;Haochi Zhang;Yanchen Liang;Ruiping Du;Xiao Wang
来自乳酸菌（LAB）的胞外多糖由于其健康益处而受到更多关注。大多数关于LAB EPS的研究集中在抗肿瘤和抗氧化活性上。据我们所知，LAB EPS的免疫佐剂活性尚未得到彻底研究。本研究采用乙醇沉淀和柱层析分离的方法对KiferiLactobacilluskiferiWXD 029胞外多糖进行了纯化。EPS的分子量为3.423 × 105 Da，主要由Glu、GlcN和GalN组成，摩尔比为3.1：1：1。体外实验表明，EPS能显著促进RAW264.7细胞的增殖和吞噬功能，并诱导NO、TNF-α、IL-1β和IL-6的产生。在体内实验中，EPS佐剂可提高金黄色葡萄球菌抗原特异性抗体的滴度，并显著促进T细胞增殖。值得注意的是，EPS佐剂还诱导了强的潜在Th 1、Th 2和Th 17细胞混合物应答。此外，在小鼠菌血症、肺炎和乳腺炎模型中，与单独的金黄色葡萄球菌抗原相比，用金黄色葡萄球菌抗原加EPS佐剂免疫诱导了保护作用。总之，这些结果表明，来源于益生菌Kiferi乳杆菌菌株的EPS是有希望作为预防S.金黄色葡萄球菌感染
Exopolysaccharides from lactic acid bacteria (LAB) have gained more attention due to their health benefits. Most research on LAB EPS focuses on antitumor and antioxidant activities. To our knowledge, the immunoadjuvant activity of LAB EPS has not been thoroughly studied. In this study, the EPS produced byLactobacilluskiferiWXD029 were purified by ethanol precipitation and column chromatography fractionation. The molecular weight of the EPS was 3.423 × 105Da and was mainly composed of Glu, GlcN, and GalN in a molar ratio of 3.1:1:1. In vitro, EPS could significantly enhance the proliferation and phagocytic activity as well as induce the production of NO, TNF-α, IL-1β, and IL-6 in RAW264.7 cells. In vivo, the EPS adjuvant could increase the titers ofS.aureusantigen-specific antibodies and markedly enhanced T cell proliferation. Notably, EPS adjuvant also induced a strong potential Th1, Th2 and Th17-cell mixture responses. Furthermore, immunization withS.aureusantigen plus EPS adjuvant induced a protective effect when compared withS.aureusantigen alone in murine bacteremia, pneumonia and mastitis model. Collectively, these results suggest that EPS derived from probioticLactobacillus kiferistrain is promising as an efficient adjuvant candidate for the prevention ofS. aureusinfections.