Acinetobacter baumannii Fatty Acid Desaturases Facilitate Survival in Distinct Environments
Acinetobacter baumannii Fatty Acid Desaturases Facilitate Survival in Distinct Environments
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DOI：
10.1021/acsinfecdis.1c00192
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发表时间：
2021-06-08
影响因子：
5.3
通讯作者：
Eijkelkamp, Bart A.
中科院分区：
文献类型：
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作者：
Adams, Felise G.;Pokhrel, Alaska;Eijkelkamp, Bart A.
在不同的环境条件下，保持最佳的流动性对于确保膜的结构和功能是至关重要的。我们应用集成的分子方法对鲍曼不动杆菌中的两种去饱和酶(DesA和DesB)进行了表征，并确定了它们在不饱和脂肪酸(UFA)生产中的具体作用。使用小鼠模型，我们发现DESA在呼吸道的定植中起着次要的作用，而DESA在侵袭性疾病中起着重要的作用。此外，利用转录组分析和生物信息学分析，对参与脂肪酸动态平衡的全球调控因子及其调节子成员进行了表征。总的来说，我们证明DESA和DesB是鲍曼不动杆菌不饱和脂肪酸产生的主要贡献者，感染研究表明这些不同的去饱和酶有助于细菌在多个宿主生态位中生存的能力。因此，这项研究为鲍曼不动杆菌脂类生物学的基本原理提供了新的见解，有助于这种关键细菌病原体的多功能性。
Maintaining optimal fluidity is essential to ensure adequate membrane structure and function under different environmental conditions. We apply integrated molecular approaches to characterize two desaturases (DesA and DesB) and define their specific roles in unsaturated fatty acid (UFA) production in Acinetobacter baumannii. Using a murine model, we reveal DesA to play a minor role in colonization of the respiratory tract, whereas DesB is important during invasive disease. Furthermore, using transcriptomic and bioinformatic analyses, a global regulator involved in fatty acid homeostasis and members of its regulon are characterized. Collectively, we show that DesA and DesB are primary contributors to UFA production in A. baumannii with infection studies illustrating that these distinct desaturases aid in the bacterium's ability to survive in multiple host niches. Hence, this study provides novel insights into the fundamentals of A. baumannii lipid biology, which contributes to the versatility of this critical bacterial pathogen.