Immunocytochemical reactivity of Xenopus laevis retinal rods and cones with several monoclonal antibodies to visual pigments
Immunocytochemical reactivity of Xenopus laevis retinal rods and cones with several monoclonal antibodies to visual pigments
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非洲爪蟾视网膜视杆细胞和视锥细胞与几种视色素单克隆抗体的免疫细胞化学反应
DOI：
10.1002/cne.902900107
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发表时间：
1989
影响因子：
2.5
通讯作者：
D. Papermaster
中科院分区：
文献类型：
--
作者：
P. Röhlich;Á. Szél;D. Papermaster
使用几种视觉色素抗体的免疫细胞化学反应来研究非洲爪蟾视网膜的视觉细胞。将牛视蛋白“E”︁ 1D4 和 4B4（分别与 N 端和 C 端以及连接跨膜片段 5-6 的环反应）和鸡视觉色素 COS-1 和 OS-2（分别与哺乳动物红/绿和蓝色视锥细胞结合）以及兔抗青蛙视蛋白血清 11-7 的单克隆抗体应用于半薄和薄切片视网膜。使用过氧化物酶技术在光学显微镜水平上检测结合的抗体；三阶段免疫金程序用于电子显微镜免疫细胞化学。
Immunocytochemical reactions with several antibodies to visual pigments were used to study visual cells of the Xenopus laevis retina. Monoclonal antibodies to bovine opsin „E,”︁ 1D4, and 4B4 (reactive with the N‐ and C‐terminus and with the loop connecting transmembrane segments 5–6, respectively) and to chicken visual pigments COS‐1 and OS‐2 (binding to mammalian red/green and blue cones, respectively), as well as a rabbit antifrog opsin serum 11–7, were applied to semithin and thin sections of the retina. The bound antibodies were detected with the peroxidase technique at the light microscopic level; a three‐stage immunogold procedure was used for electron microscopic immunocytochemistry.
使用单克隆抗体在视杆感光盘膜中组织视紫红质和高分子量糖蛋白。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
MacKenzie,D;Molday,RS
通讯作者：
Molday,RS
影响因子：
3.4
作者：
HICKS, D;MOLDAY, RS
通讯作者：
MOLDAY, RS