In Vivo Bioluminescence Imaging of Cobalt Accumulation in a Mouse Model
In Vivo Bioluminescence Imaging of Cobalt Accumulation in a Mouse Model
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小鼠模型中钴积累的体内生物发光成像
DOI：
10.1021/acs.analchem.8b00391
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发表时间：
2018
影响因子：
7.4
通讯作者：
Li Minyong
中科院分区：
文献类型：
--
作者：
Ke Bowen;Ma Lin;Kang Ting;He Wei;Gou Xueyan;Gong Deying;Du Lupei;Li Minyong
钴作为一种微量营养元素，对原核生物和真核生物都至关重要。在当前的研究中，基于氧化C-O键断裂成功开发了用于检测钴的开启型钴生物发光探针1（CBP-1）。该探针对钴的选择性和灵敏度高于其他分析物。利用CBP-1在小鼠模型中成功地进行了钴蓄积的体内显像。这种在活体动物中测定钴的能力为研究Co ~（2+）的系统分布、毒性和生物效应提供了强有力的技术。
As a trace element nutrient, cobalt is critical for both prokaryotes and eukaryotes. In the current study, a turn-on Cobalt Bioluminescent Probe 1(CBP-1)for the detection of cobalt has been successfully developed based on oxidative C–O bond cleavage. This probe exhibited high selectivity and sensitivity toward cobalt over other analytes. By usingCBP-1, the successfulin vivoimaging of cobalt accumulation was carried out in a mouse model. Such an ability to determine cobalt in living animals provides a powerful technology for studying the system distribution, toxic potency, and biological effect of Co2+.