Intracerebral Delivery of an OTR Antagonist and Subsequent Behavioral Testing in Prairie Voles
Intracerebral Delivery of an OTR Antagonist and Subsequent Behavioral Testing in Prairie Voles
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草原田鼠脑内注射 OTR 拮抗剂及后续行为测试
DOI：
10.1007/978-1-0716-1759-5_16
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Mitsui Shinichi
中科院分区：
文献类型：
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作者：
Hirota Yu;Otsuki Chisato;Kamura Erika;Mitsui Shinichi
啮齿动物的行为学分析在当前的神经科学研究中是不可或缺的。与传统的实验啮齿动物不同，草原田鼠(Microtus Ochrogaster)是一夫一妻制的，比起陌生的田鼠，它们更喜欢与社会性伴侣挤在一起。草原田鼠的伴侣偏好使用三个透明的小室进行评估。左右腔室通过中空管与中央腔室相连。一只异性伙伴田鼠被拴在右室或左室，而一只陌生的田鼠被拴在另一室。一只被试草原田鼠被放置在中央房间，并允许在三个房间自由漫步3个小时。行为被记录在房间前放置的录像机中。与伙伴和陌生田鼠挤在一起的持续时间是以盲目方式手工评分的。在这次的伴侣偏好测试中，亚克力室和其他材料都是定制的。在这里，我们描述这些设备的详细规格。此外，我们还提到了插管植入和脑室注射的方案，以及草原田鼠被动回避测试的标准方案。
Ethological analyses of rodents are indispensable in current neuroscience research. Unlike traditional experimental rodents, prairie voles (Microtus ochrogaster) are monogamous, preferring to huddle with their sociosexual partner rather than stranger voles. Partner preference in prairie voles is assessed using three transparent chambers. The right and left chambers are connected with the central chamber via hollow tubes. An opposite-sex partner vole is tethered to the right or left chamber, while a stranger vole is tethered to the other one. A subject prairie vole is placed in the central chamber and allowed to roam freely in the three chambers for 3 h. Behaviors are recorded in a video recorder placed in front of the chambers. The duration to huddle with the partner and stranger voles is hand-scored in a blinded fashion. In this partner preference test, acrylic chambers and other materials are custom-made. Here, we describe detailed specification of these apparatuses. In addition, we mention protocols for cannula implantation and intracerebroventricular injection as well as a standard protocol of a passive avoidance test in prairie voles.