Characterization of circular RNAs landscape in multiple system atrophy brain
Characterization of circular RNAs landscape in multiple system atrophy brain
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DOI：
10.1111/jnc.13752
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发表时间：
2016-11-01
影响因子：
4.7
通讯作者：
Janitz, Michael
中科院分区：
文献类型：
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作者：
Chen, Bei Jun;Mills, James D.;Janitz, Michael
环状RNA（CircRNA）是近年来发现的一类广泛存在的内源性非编码RNA家族，可调控哺乳动物的基因表达。它们是异常稳定的RNA分子，具有细胞类型或发育阶段特异性表达模式。然而，circRNA在复杂疾病的病理学中的作用是完全未知的。在这里，我们报告特定的环状转录组在多系统萎缩（MSA）的大脑中确定的RNA测序。五个circRNA，即IQCK，MAP 4K 3，EFCAB 11，DTNA和MCTP 1，被鉴定和验证为在MSA额叶皮层中特异性过表达。线性转录本的表达水平没有显著改变，因此不遵循其环状对应物的模式。对五种MSA特异性circRNA表达的进一步分析揭示了它们在MSA皮质组织的白色物质中的过表达。总之，这是第一份报告描述干扰的α-突触核蛋白病的环状转录组。
Circular RNAs (circRNAs) have been recently identified as a naturally occurring family of widespread and diverse endogenous non-coding RNAs that may regulate gene expression in mammals. They are unusually stable RNA molecules with cell type-or developmental stage-specific expression patterns. However, the role of circRNAs in pathology of complex disease is entirely unknown. Here, we report the specific circular transcriptome in the multiple system atrophy (MSA) brain as determined by RNA sequencing. Five circRNAs, namely IQCK, MAP4K3, EFCAB11, DTNA, and MCTP1, were identified and validated as specifically over-expressed in MSA frontal cortex. The expression levels of linear transcripts were not significantly altered and thus did not follow the pattern of their circular counterparts. Further analysis of expression of five MSA-specific circRNAs revealed their over-expression in the white matter of the MSA cortical tissue. Together, this is the first report describing perturbation of circular transcriptome in alpha-synucleinopathies.