Antifungal and Antibiofilm In Vitro Activities of Ursolic Acid on Cryptococcus neoformans
Antifungal and Antibiofilm In Vitro Activities of Ursolic Acid on Cryptococcus neoformans
复制标题
DOI：
10.1007/s00284-022-02992-5
复制
发表时间：
2022-08
影响因子：
2.6
通讯作者：
Wenjing Wang;Chan-Chan Liu-Chan;Yan-Tong Li;Miaorong Li;Yuting Fu;Xin-Chen Li;Jie-Kang;Weidong Qian
中科院分区：
文献类型：
--
作者：
Wenjing Wang;Chan-Chan Liu-Chan;Yan-Tong Li;Miaorong Li;Yuting Fu;Xin-Chen Li;Jie-Kang;Weidong Qian
熊果酸（UA）存在于多种药用植物中。UA对几种微生物具有抗菌活性；然而，关于UA对新型隐球菌的潜在抗真菌作用知之甚少。neoformans)。UA onC的抗真菌和抗膜活性。在本研究中对sh99进行了评估。最低抑菌浓度（MIC）。通过微量稀释技术测定neoformsh99，并通过阐明细胞膜完整性、胶囊和黑色素生成的变化来阐明其作用模式。此外，还研究了UA对生物膜形成和成熟生物膜的抑制和扩散作用。采用结晶紫（CV）测定、光学显微镜、场发射扫描电镜和共聚焦激光扫描显微镜对neoophomonsh99进行评价。结果表明，UA对c的MIC值。neoformsh99为0.25 mg/mL。UA破坏了细胞膜的完整性，抑制了c的包膜和黑色素的产生。以浓度依赖的方式。此外，UA对生物膜的形成和成熟生物膜的分散有抑制作用，并破坏了c细胞膜的完整性。生物膜内的新生sh99细胞。总之，这些结果表明UA可能是治疗ofC的潜在治疗选择。neoformans-related感染。
Ursolic acid (UA) exists in a variety of medicinal plants. UA exhibits antimicrobial activity against several microorganisms; however, little is known regarding the potential antifungal effect of UA onCryptococcus neoformans(C. neoformans). The antifungal and antibiofilm activities of UA onC. neoformansH99 were evaluated in this study. Minimum inhibitory concentration (MIC) of UA againstC. neoformansH99 was determined by microdilution technique, and its action mode was elucidated by clarifying the variations in cell membrane integrity, capsule, and melanin production. Moreover, the inhibition and dispersal effects of UA on biofilm formation and mature biofilms byC. neoformansH99 were evaluated using crystal violet (CV) assay, optical microscopy, field emission scanning electron microscopy and confocal laser scanning microscopy. The results indicated that the MIC value of UA againstC. neoformansH99 was 0.25 mg/mL. UA disrupted the cell membrane integrity, inhibited the capsule and melanin production ofC. neoformansH99 in a concentration-dependent manner. Further, UA presented the inhibitory effect on biofilm formation and dispersed mature biofilms, as well as compromised the cell membrane integrity ofC. neoformansH99 cells within biofilms. Together, these results indicate that UA might be a potential therapeutic option for the treatment ofC. neoformans-related infections.