Polymerase I and transcript release factor acts as an essential modulator of glioblastoma chemoresistance.
Polymerase I and transcript release factor acts as an essential modulator of glioblastoma chemoresistance.
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聚合酶 I 和转录释放因子是胶质母细胞瘤化疗耐药性的重要调节剂
DOI：
10.1371/journal.pone.0093439
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Guo H
中科院分区：
文献类型：
--
作者：
Wang X;Liu T;Bai Y;Liao H;Qiu S;Chang Z;Liu Y;Yan X;Guo H
目的 本研究旨在探讨聚合酶 I 和转录释放因子 (PTRF) 是否充当胶质母细胞瘤 (GBM) 化疗耐药性的调节剂。方法通过将U251细胞系暴露于伊马替尼建立多药耐药（MDR）GBM细胞系U251AR。对 U251 和 U251AR 细胞系进行 2D-DIGE 和 MALDI-TOF/TOF-MS 筛选 MDR 相关蛋白。通过蛋白质印迹和定量 RT-PCR 分析确定 PTRF 的表达。结果与亲本U251细胞相比，U251AR细胞中21种蛋白的表达发生显着改变。在21个差异表达蛋白中，与亲本U251细胞相比，U251AR细胞中PTRF的表达上调了2.14倍。 GBM细胞系中PTRF的敲低显着增加了细胞对各种化学药物的化学敏感性，并降低了caveolae的主要结构成分caveolin1的表达水平。复发性 GBM 患者中 PTRF 和 Caveolin1 的表达水平显着上调。同一GBM标本中PTRF和caveolin1的mRNA水平呈正相关。结论 我们的结果表明 PTRF 作为 GBM 化疗耐药性的调节剂。
Objectives This study is to investigate if polymerase I and transcript release factor (PTRF) acts as a modulator in glioblastoma (GBM) chemoresistance. Methods Multidrug resistant (MDR) GBM cell line U251AR was established by exposing the U251 cell line to imatinib. The 2D-DIGE and MALDI-TOF/TOF-MS were performed on U251 and U251AR cell lines to screen MDR-related proteins. The expression of PTRF was determined by Western blot and quantitative RT-PCR analyses. Results When compared with the parental U251 cells, expression of 21 proteins was significantly altered in U251AR cells. Among the 21 differentially expressed proteins, the expression of PTRF was up-regulated by 2.14 folds in U251AR cells when compared with that in the parental U251 cells. Knockdown of PTRF in GBM cell lines significantly increased chemosensitivity of cells to various chemical drugs and decreased the expression levels of caveolin1, a major structural component of caveolae. Expression levels of PTRF and caveolin1 were significantly up-regulated in the relapsed GBM patients. The mRNA level of PTRF and caveolin1 showed a positive correlation in the same GBM specimens. Conclusions Our results indicate that PTRF acts as a modulator in GBM chemoresistance.
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DOI：
10.1083/jcb.200903053
发表时间：
2009-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bastiani M;Liu L;Hill MM;Jedrychowski MP;Nixon SJ;Lo HP;Abankwa D;Luetterforst R;Fernandez-Rojo M;Breen MR;Gygi SP;Vinten J;Walser PJ;North KN;Hancock JF;Pilch PF;Parton RG
通讯作者：
Parton RG
影响因子：
8.4
作者：
Bai, Yifeng;Liao, Hongzhan;Guo, Linlang
通讯作者：
Guo, Linlang
影响因子：
2.8
作者：
Gould, M. L.;Williams, G.;Nicholson, H. D.
通讯作者：
Nicholson, H. D.
影响因子：
3.4
作者：
Kumar, Durairaj M.;Patil, Vikas;Somasundaram, Kumaravel
通讯作者：
Somasundaram, Kumaravel
影响因子：
3.7
作者：
Gámez-Pozo A;Sánchez-Navarro I;Calvo E;Agulló-Ortuño MT;López-Vacas R;Díaz E;Camafeita E;Nistal M;Madero R;Espinosa E;López JA;Fresno Vara JÁ
通讯作者：
Fresno Vara JÁ