Human apolipoproteins AI, AII, CII and CIII. cDNA sequences and mRNA abundance.
Human apolipoproteins AI, AII, CII and CIII. cDNA sequences and mRNA abundance.
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人类载脂蛋白 AI、AII、CII 和 CIII。
DOI：
10.1093/nar/12.9.3917
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发表时间：
1984
影响因子：
14.9
通讯作者：
F. Baralle
中科院分区：
文献类型：
--
作者：
C. Sharpe;A. Sidoli;C. Shelley;M. Lucero;C. Shoulders;F. Baralle
编码血浆脂蛋白多肽成分的基因的结构和功能引起了人们的兴趣，因为它们在脂质代谢的调节中起着核心作用。目前，我们已经完成了前期对人类apoAI基因的研究，并进一步分离和测序了apoAII、CII和CIII的cDNA克隆。核苷酸序列显示，apoAII、CII和CIII的信号肽长度分别为18、22和20个氨基酸，此外，前apoAII具有5个氨基酸的经典前肽结构。讨论了apoCII和前apoAI之间的氨基酸同源性，以及apoAI mRNA的5'非编码区基因的排列。本研究中分析的4种载脂蛋白的相对肝脏mRNA水平已被估计并与其相应的血浆产物进行了比较。本文报道的数据为进一步研究载脂蛋白AI、AII、CII和CIII基因的结构和功能等位基因提供了必要的基础。
The structure and function of the genes encoding the polypeptide components of plasma lipoproteins are of interest because of the central role they play in the regulation of lipid metabolism. We have now completed our previous studies on the human apoAI gene and furthermore isolated and sequenced cDNA clones for apoAII , CII and CIII. The nucleotide sequences show the signal peptides of apoAII , CII and CIII to be 18, 22 and 20 amino acids in length, respectively, and in addition that prepro apoAII bears a classical propeptide structure of 5 amino acids. The amino acid homology detected between apoCII and pro- apoAI is discussed, as is the gene arrangement of the 5' non-coding region of apoAI mRNA. The relative liver mRNA levels of the 4 apolipoproteins analysed in this study have been estimated and compared with their corresponding plasma products. The data reported here provide an essential basis for further studies of structural and functional alleles of apo AI, AII, CII and CIII genes.