A Unique Fluorescent Base Analogue for the Expansion of the Genetic Alphabet
A Unique Fluorescent Base Analogue for the Expansion of the Genetic Alphabet
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DOI：
10.1021/ja100806c
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发表时间：
2010-04-14
影响因子：
15
通讯作者：
Hirao, Ichiro
中科院分区：
文献类型：
--
作者：
Kimoto, Michiko;Mitsui, Tsuneo;Hirao, Ichiro
荧光核碱基类似物在各种生物学和生物技术工具中作为分子探针和核酸报告者是有用的。在这里，我们提出了一种新的荧光嘌呤类似物，7-(2,2'-bithien-5-yl)-咪唑[4,5-b]吡啶（记为Dss）。Dss的核苷三磷酸可以通过聚合酶位点特异性地结合到DNA和RNA中，与DNA模板中的配对伙伴吡咯-2-乙醛（Pa）相反。尽管它在复制和转录方面具有很高的特异性，但寡核苷酸中的Dss作为一种通用碱基，与所有四种天然碱基配对，具有几乎相同的热稳定性。因此，Dss将成为在功能性DNA和RNA分子的特定位置进行荧光碱基替换的有力工具。
Fluorescent nucleobase analogues are useful in a wide variety of biology and biotechnology tools as molecular probes and reporters for nucleic acids. Here we present a novel fluorescent purine analogue, 7-(2,2'-bithien-5-yl)-imidazo[4,5-b]pyridine (denoted as Dss). The nucleoside triphosphates of Dss can be site-specifically incorporated into DNA and RNA by polymerases, opposite its pairing partner, pyrrole-2-carbaldehyde (Pa), in DNA templates. Despite its high specificity in replication and transcription, Dss in oligonucleotides functions as a universal base that pairs with all four natural bases with nearly equal thermal stabilities. Thus, Dss would be a powerful tool for fluorescent base replacements at specific positions in functional DNA and RNA molecules.