Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation.
Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation.
复制标题
配子分化缺陷的莱茵衣藻突变株的分离和遗传分析。
作者：
U. Goodenough;C. Hwang;H. Martin
本文描述了莱因哈氏衣原体交配型（mt）+的无能突变株，其具有正常的生长和运动性，但不能分化成正常的配子。它们的分离和遗传分析的程序进行了说明。当与mt-或mt+配子混合时，五种imp菌株（imp-2、imp-5、imp-1、imp-7和imp-8）未表现出鞭毛凝集，并且突变显示与mt基因座无关（imp-7可能例外）。其中两个菌株（imp-3和imp-4）携带影响细胞融合的泄漏突变;通过四分体分析发现，这两个突变都不与mt或另一个突变相关。imp-1菌株的细胞与mt-配子凝集良好，并且活性凝集持续长达48小时，但细胞融合仅极少数发生。对这些罕见合子的分析表明，imp-1与mt+位点紧密连锁，精细结构研究表明，imp-1配子产生一种参与合子细胞融合的突变交配结构。C.因此，莱因哈迪似乎可以进行基因解剖。
Impotent mutant strains of Chlamydomonas reinhardi, mating-type (mt) plus, are described that have normal growth and motility but fail to differentiate into normal gametes. Procedures for their isolation and their genetic analysis are described. Five of the imp strains (imp-2, imp-5, imp-l, imp-7, and imp-8) exhibit no flagellar agglutination when mixed with mt- or mt+ gametes and the mutations are shown to be unlinked to the mt locus (with the possible exception of imp-7). Two of the strains (imp-3 and imp-4) carry leaky mutations that affect cell fusion; neither mutation is found by tetrad analysis to be linked to mt or to the other. Cells of the imp-1 strain agglutinate well with mt- gametes and active agglutination continues for up to 48 hours, but cell fusion occurs only very rarely. Analysis of these rare zygotes indicates that imp-1 is closely linked to the mt+ locus, and fine-structural studies reveal that imp-1 gametes produce a mutant mating structure involved in zygotic cell fusion. The development of sexuality in C. reinhardi therefore appears amenable to genetic dissection.