Serotonin produces long-term changes in the excitability of Aplysia sensory neurons in culture that depend on new protein synthesis
Serotonin produces long-term changes in the excitability of Aplysia sensory neurons in culture that depend on new protein synthesis
复制标题
血清素对培养的海兔感觉神经元的兴奋性产生长期变化，这取决于新的蛋白质合成
DOI：
10.1523/jneurosci.07-07-02232.1987
复制
发表时间：
1987
影响因子：
1.8
通讯作者：
S. Schacher
中科院分区：
文献类型：
--
作者：
N. Dale;E. Kandel;S. Schacher
分离培养后，鳃退缩反射的感觉神经元和运动神经元很容易形成突触连接。反复暴露于5-HT可促进共培养感觉神经元和运动神经元之间的突触连接，持续至少24小时。作为理解这种长期突触促进的位点和机制的第一步，我们研究了分离的突触前感觉神经元在分离细胞培养中单独生长的膜兴奋性。4次1微米5-HT的重复应用导致感觉神经元的兴奋性显著增加，持续至少24小时。这类似于单次使用5-羟色胺后兴奋性的短期变化。与短期效果不同，这种长期的变化被5-HT处理期间细胞暴露于10微米的大尼索霉素（一种蛋白质合成抑制剂）所阻断。因此，与突触易化一样，分离的突触前神经元兴奋性的长期变化与需要合成新蛋白的短期变化不同。这一发现表明，感觉神经元使用基因产物来调节膜电流，这是对重复外部刺激的长期反应，而不是对单一刺激的短期反应所需要的。
When isolated and grown in cell culture, the sensory and motor neurons of the gill withdrawal reflex of Aplysia readily form synaptic connections. Repeated exposures to 5-HT cause facilitation of the synaptic connections between co-cultured sensory and motor neurons lasting at least 24 hr. As a first step toward understanding the locus and the mechanisms underlying this long-term synaptic facilitation, we have examined the membrane excitability of the isolated presynaptic sensory neurons grown alone in dissociated cell culture. Four repeated applications of 1 microM 5-HT caused a significant increase in the excitability of sensory neurons, lasting at least 24 hr. This resembles the short-term changes in excitability seen in response to a single application of 5-HT. Unlike the short-term effect, this long-lasting change was blocked by exposure of the cells during the 5-HT treatment to 10 microM anisomycin, an inhibitor of protein synthesis. Thus, like the synaptic facilitation, the long-term change in excitability of the isolated presynaptic neurons differs from the short-term in requiring the synthesis of new protein. This finding suggests that the sensory neuron uses gene products to modulate membrane currents in its long- term response to repeated external stimuli that are not required in its short-term response to a single stimulus.