Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions.
Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions.
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低温条件下 DHA 生产者 Aurantiochytrium 的转录组和基因表达分析。
DOI：
10.1038/srep14446
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发表时间：
2015-09-25
影响因子：
4.6
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Ma Z;Tan Y;Cui G;Feng Y;Cui Q;Song X
金氧化钇因其生长速度快、脂质和DHA含量高而成为很有前途的二十二碳六烯酸（DHA）生产候选植物。本研究采用高通量RNA测序技术，在转录水平上探讨了这种DHA生产者在冷胁迫下的驯化过程。整个cDNA序列数据的从头组装产生29,783个单基因，平均长度为1,200 bp。总共有13245个unigenes在至少一个数据库中被注释。通过对正常和冷胁迫条件下的基因组比较分析发现，在生长阶段有2013个基因存在差异表达，而在脂质积累阶段有2071个基因存在差异表达。进一步的功能分类和分析表明，一些差异表达基因参与了冷驯化的关键过程，如信号转导、细胞成分生物发生、碳水化合物和脂质代谢。对在冷胁迫下获得的转录本的简要调查强调了Aurantiochytrium的生存策略；在这些转录本中，许多直接或间接影响脂质组成。这是第一个在低温条件下对金锑钇进行转录组学分析的研究。我们的研究结果将有助于在未来提高金氧化钇的DHA产量。
Aurantiochytrium is a promising docosahexaenoic acid (DHA) production candidate due to its fast growth rate and high proportions of lipid and DHA content. In this study, high-throughput RNA sequencing technology was employed to explore the acclimatization of this DHA producer under cold stress at the transcriptional level. The overall de novo assembly of the cDNA sequence data generated 29,783 unigenes, with an average length of 1,200 bp. In total, 13,245 unigenes were annotated in at least one database. A comparative genomic analysis between normal conditions and cold stress revealed that 2,013 genes were differentially expressed during the growth stage, while 2,071 genes were differentially expressed during the lipid accumulation stage. Further functional categorization and analyses showed some differentially expressed genes were involved in processes crucial to cold acclimation, such as signal transduction, cellular component biogenesis, and carbohydrate and lipid metabolism. A brief survey of the transcripts obtained in response to cold stress underlines the survival strategy of Aurantiochytrium; of these transcripts, many directly or indirectly influence the lipid composition. This is the first study to perform a transcriptomic analysis of the Aurantiochytrium under low temperature conditions. Our results will help to enhance DHA production by Aurantiochytrium in the future.