Modulation of murine neuroblastoma in nude mice by opioid antagonists.
Modulation of murine neuroblastoma in nude mice by opioid antagonists.
复制标题
阿片类拮抗剂对裸鼠神经母细胞瘤的调节作用。
DOI：
10.1093/jnci/78.1.141
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
McLaughlin,PJ
中科院分区：
文献类型：
--
作者：
Zagon,IS;McLaughlin,PJ
纳曲酮是一种阿片拮抗剂，对BALB/c裸鼠体内的鼠S20Y神经母细胞瘤的生长有抑制作用。每天注射 0.1 毫克纳曲酮/公斤，每天注射 6-8 小时的受体阻断剂，导致肿瘤表达前的潜伏时间延迟 31-92%，平均生存时间增加 27-49%；抗肿瘤反应的强度取决于肿瘤负荷。接种神经母细胞瘤（106−2.5×104 个细胞）在 10–13 天内产生可测量的肿瘤，平均存活时间为 30–34 天。在肿瘤组织中检测到免疫反应性β-内啡肽（39.7 pg/mg 蛋白质）。受体结合测定揭示了与 δ 和 κ 结合位点相关的配体的特异性饱和结合，但与 μ 结合位点无关。这些结果表明阿片拮抗剂对神经肿瘤发生的调节不依赖于T细胞介导的免疫的完整性，并表明利用裸鼠模型探索内源性阿片类药物在人类癌症中的作用的可行性。
Naltrexone, an opioid antagonist, had an inhibitory effect on the growth of murine S20Y neuroblastoma in BALB/c nude mice. Daily injections of 0.1 mg naltrexone/kg, which invoked a receptor blockade for 6–8 hours/day, resulted in 31–92% delay in latency time prior to tumor expression and a 27–49% increase in mean survival time; the magnitude of antitumor response was governed by tumor burden. Inoculation of neuroblastoma (106−2.5×104cells) resulted in measurable tumors in 10–13 days and mean survival times of 30–34 days. Immunoreactive β-endorphin was detected in tumor tissue (39.7 pg/mg protein). Receptor binding assays revealed specific saturable binding of ligands related to δ- and κ-binding sites, but not for the μ-binding site. These results demonstrate that opioid antagonist modulation of neuro-oncogenesis is not dependent on the integrity of T-cell-mediated immunity and suggest the feasibility of utilizing the nude mouse model in exploring the role of endogenous opioids in human cancers.