Nanotopography reveals metabolites that maintain the immunosuppressive phenotype of mesenchymal stem cells
Nanotopography reveals metabolites that maintain the immunosuppressive phenotype of mesenchymal stem cells
复制标题
DOI：
10.1101/603332
复制
发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
Ewan Ross;L. Turner;A. Saeed;Karl E. V. Burgess;Gavin Blackburn;P. Reynolds;J. Wells;J. Mountford;N. Gadegaard;M. Salmerón-Sánchez;R. Oreffo;M. Dalby
中科院分区：
文献类型：
--
作者：
Ewan Ross;L. Turner;A. Saeed;Karl E. V. Burgess;Gavin Blackburn;P. Reynolds;J. Wells;J. Mountford;N. Gadegaard;M. Salmerón-Sánchez;R. Oreffo;M. Dalby
Mesenchymal stem cells (MSCs) are multipotent stem cells that are immunosuppressive and thus of considerable therapeutic potential in transplant operations. However, MSCs rapidly differentiate once in culture, making their large-scale expansion for use in immunosuppressive therapies challenging. Although the differentiation mechanisms of MSCs have been extensively investigated using materials, little is known about how materials can modulate paracrine activities of MSCs. Here, we show for the first time that nanotopography can control the immunomodulatory capacity of MSCs through decreased intracellular tension increasing oxidative glycolysis. We also use the nanotopography to identify bioactive metabolites that modulate intracellular tension, growth and immunomodulatory phenotype of MSCs in standard culture. Our findings show a novel route to support large-scale expansion of functional MSCs for therapeutic purposes
