METAL CHELATE AFFINITY CHROMATOGRAPHY, A NEW APPROACH TO PROTEIN FRACTIONATION
METAL CHELATE AFFINITY CHROMATOGRAPHY, A NEW APPROACH TO PROTEIN FRACTIONATION
复制标题
DOI：
10.1038/258598a0
复制
发表时间：
1975-01-01
期刊：
影响因子：
64.8
通讯作者：
BELFRAGE, G
中科院分区：
文献类型：
--
作者：
PORATH, J;CARLSSON, J;BELFRAGE, G
传统的非特异性沉淀方法有时依赖于亲和力，现代色谱技术可以以更选择性的方式使用亲和力。例如，蛋白质对重金属离子的亲和力可以为其纯化和分析提供基础。这里描述了一种基于这种亲和力的高度灵活的方法。
CONVENTIONAL nonspecific precipitation methods sometimes depend on affinities which can be used in a more selective fashion by modern chromatographic techniques. The affinity of proteins for heavy metal ions, for example, may provide a basis for their purification and analysis. A highly flexible method based on such affinities is described here.