Simultaneous Determination of Thujopsene and Cedrol in Cedarwood Oils from Thuja sutchuenensis and Platycladus orientalis by GC-MS
Simultaneous Determination of Thujopsene and Cedrol in Cedarwood Oils from Thuja sutchuenensis and Platycladus orientalis by GC-MS
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DOI：
10.1080/0972060x.2011.10643900
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发表时间：
2011-01
影响因子：
2.4
通讯作者：
Lei Huaping;Liang Feng-yin;W. Yonggang;Peng Wei;Su Weiwei;Yan Cuiping;Wang Ning
中科院分区：
文献类型：
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作者：
Lei Huaping;Liang Feng-yin;W. Yonggang;Peng Wei;Su Weiwei;Yan Cuiping;Wang Ning
柏木油的主要成分是崖柏和雪松醇，传统上用它们的含量来测定柏木油的商业价值。因此，对崖柏素和雪松醇进行定量分析非常重要。本文采用GC-MS选择离子监测（SIM）模式同时测定水蒸气蒸馏法（HD）和超临界流体萃取法（SFE）提取的崖柏（Thuja sutchuenensis）和侧柏（Platycladus orientalis）CWO中崖柏苷和雪松醇的含量。侧柏叶素和雪松醇在0.99-495.25、0.61 ~ 300.50 μg/ml范围内线性关系良好，定量限分别为0.2、0.4 ng/ml。该方法也表现出良好的重复性，分别为0.59%和1.74%，日内变异和日间变异分别为0.99%和3.06%，用于定量侧柏叶素和雪松醇。该方法已成功应用于中国红豆杉CWO中侧柏叶素和雪松醇的定量分析。sutchuenensis和P. orientalis。该分析方法也可用于定量来自柏科其他物种的CWO中的崖柏苷和雪松醇。
Abstract Thujopsene and cedrol are the main components of cedarwood oil (CWO) and their concentrations have been traditionally used to determine the oil’s commercial value. Therefore, quantification of thujopsene and cedrol is very important. In this paper, GC-MS in selected ion monitoring (SIM) mode was developed to determine simultaneously thujopsene and cedrol in CWOs from Thuja sutchuenensis and Platycladus orientalis extracted by hydrodistillation (HD) and supercritical fluid extraction (SFE). Thujopsene and cedrol showed excellent linearity in the range of 0.99–495.25, 0.61∼300.50 μg/ml and the limits of quantification were 0.2, 0.4 ng/ml, respectively. The method also showed good repeatability for quantification of thujopsene and cedrol with intra-day variations of 0.59 and 1.74 %, respectively, and inter-day variations of 0.99 and 3.06 %, respectively. The validated method was successfully applied to quantify thujopsene and cedrol in CWOs from T. sutchuenensis and P. orientalis. The analytical method can also be used to quantify thujopsene and cedrol in CWOs from other species of the family Cupressaceae.