Isolation and characterization of an oxygen radical absorbance activity peptide from defatted peanut meal hydrolysate and its antioxidant properties.
Isolation and characterization of an oxygen radical absorbance activity peptide from defatted peanut meal hydrolysate and its antioxidant properties.
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DOI：
10.1021/jf3017173
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发表时间：
2012-05
影响因子：
6.1
通讯作者：
Lin Zheng;G. Su;Jiaoyan Ren;Longjian Gu;Lijun You;Mouming Zhao
中科院分区：
文献类型：
--
作者：
Lin Zheng;G. Su;Jiaoyan Ren;Longjian Gu;Lijun You;Mouming Zhao
采用超滤、凝胶过滤色谱和高效液相色谱法对脱脂花生粕水解液（DPMH）进行纯化。分离得到一种具有强氧自由基吸收能力（ORAC）的三肽，经ESI-MS/MS鉴定为Tyr-Gly-Ser。Tyr-Gly-Ser的ORAC值是GSH的3倍，对亚油酸过氧化和H2 O2诱导的大鼠嗜铬细胞瘤细胞系PC 12细胞氧化损伤的保护作用强于GSH（p < 0.05）。然而，Tyr-Gly-Ser表现出可忽略的DPPH自由基清除活性，还原能力，并且没有金属螯合能力。结果表明，Tyr-Gly-Ser通过氢原子转移机制表现出抗氧化活性，N端的Tyr为氢供体。在抗氧化肽的分离过程中，ORAC法是一种可靠、有效的抗氧化活性测定方法。
Defatted peanut meal hydrolysate (DPMH) was purified using ultrafiltration, gel filtration chromatography, and high-performance liquid chromatography. A tripeptide with strong oxygen radical absorbance capacity (ORAC) was isolated and identified as Tyr-Gly-Ser by ESI-MS/MS. It was then synthesized to measure its antioxidant properties in different systems. The ORAC value of Tyr-Gly-Ser was 3-fold higher than that of glutathione (GSH), and it displayed a stronger protective effect on linoleic acid peroxidation and H(2)O(2)-induced oxidative injury in rat pheochromocytoma line PC12 cells than GSH (p < 0.05). However, Tyr-Gly-Ser showed negligible DPPH radical scavenging activity, reducing power, and no metal chelating ability. The results suggested that Tyr-Gly-Ser displayed antioxidant activity via the hydrogen atom transfer mechanism, and the Tyr at the N-terminal was the hydrogen donor. The ORAC assay was recommended as a reliable and effective method to measure the antioxidant activity in the course of antioxidant peptide isolation.