Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting analyses via colored stacking gels
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting analyses via colored stacking gels
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DOI：
10.1016/j.ab.2022.114751
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发表时间：
2022-06-06
影响因子：
2.9
通讯作者：
Hagiwara，Makoto
中科院分区：
文献类型：
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作者：
Hagiwara，Makoto
十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 是蛋白质分析的标准方法。然而，标准 SDS-PAGE 所使用的浓缩胶是透明且模糊的。因此，将样品加载到堆积凝胶孔中可能具有挑战性。因此，将一种酸性染料（柠檬黄、亮蓝 FCF 或新的 coccine）添加到 SDS-PAGE 浓缩胶中来解决这个问题，这种染料可以轻松可视化浓缩胶孔并直接将样品加载到这些孔中。此外，这些凝胶的性能与非彩色标准凝胶相当。因此，全世界的研究人员都可以采用这种简单的方法进行蛋白质分析。
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a standard method for protein analysis. However, the stacking gel employed for the standard SDS-PAGE is transparent and indistinct. Therefore, loading samples into stacking gel wells can be challenging. Accordingly, an acidic dye (tartrazine, brilliant blue FCF, or new coccine), which allowed easy visualization of the stacking gel wells and straightforward sample loading into these wells, was added to the SDS-PAGE stacking gel to resolve this issue. Moreover, the performance of these gels was comparable to that of non-colored standard gels. Thus, researchers worldwide could adopt this simple method for protein analysis.