Induction of recessive lethal mutations in the T/t-H-2 region of the mouse genome by a point mutagen.
Induction of recessive lethal mutations in the T/t-H-2 region of the mouse genome by a point mutagen.
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通过点诱变剂在小鼠基因组的 T/t-H-2 区域诱导隐性致死突变。
DOI：
10.1017/s0016672300022977
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Dove,WF
中科院分区：
文献类型：
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作者：
Shedlovsky,A;Guenet,JL;Johnson,LL;Dove,WF
小鼠17号染色体上的T/t-H-2区域是已知的复杂的天然变异体（“t-单倍型”），包含许多基因，包括一些影响免疫系统和胚胎发育的基因。分离这50兆碱基对区域的重组DNA克隆的快速进展正在为其完整的分子解剖产生材料。揭示该区域控制的生物学功能的关键一步是获得基因单独失活的突变体。我们使用一对近交系小鼠品系和一系列经典的育种计划，允许检测隐性致死和有害突变的T/t-H-2区域。在我们的研究的初始阶段，280配子诱变的雄性生殖系中的乙基亚硝基脲（ENU）产生了11个独立的产前隐性致死突变。四个已被映射到T突变，并已被证明在所有成对组合中彼此互补。
The T/t–H-2 region on mouse chromosome 17 is known from complex natural variants (‘t-haplotypes’) to contain numerous genes, including some affecting the immune system and the development of the embryo. Rapid progress in the isolation of recombinant DNA clones for this 50 megabasepair region is generating the material for its complete molecular anatomy. A crucial step in revealing the biological functions controlled by the region is to obtain mutants in which genes are inactivated individually. We have used a pair of inbred mouse strains and a series of classical breeding schemes that permit the detection of recessive lethal and detrimental mutations in the T/t–H-2 region.In this initial phase of our study, 280 gametes mutagenized in the male germ line by ethylnitrosourea (ENU) have yielded eleven independent pre-natal recessive lethal mutations. Four have been mapped against T mutations and have been shown to complement one another in all pairwise combinations.