sCD163 in AOSD: a biomarker for macrophage activation related to hyperferritinemia
sCD163 in AOSD: a biomarker for macrophage activation related to hyperferritinemia
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DOI：
10.1007/s12026-014-8563-7
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发表时间：
2014-12-01
影响因子：
4.4
通讯作者：
Valesini, G.
中科院分区：
文献类型：
--
作者：
Colafrancesco, S.;Priori, R.;Valesini, G.
铁蛋白在成人斯蒂尔病（AOSD）中起关键作用。它的产生似乎与巨噬细胞活化有关，而sCD 163是巨噬细胞活化的主要血清标志物。因此，我们旨在评估sCD 163在AOSD中的作用及其与铁蛋白的关系。此外，我们确定了表达的CD 163和铁蛋白的淋巴结从AOSD患者。检测了34例AOSD患者（21例活动期，13例非活动期）、18例脓毒症患者和22例健康对照者（HC）的sCD 163和血清铁蛋白。对来自AOSD患者的淋巴结进行免疫组织学检查，以检测CD 163和铁蛋白。来自HC的扁桃体用作对照。活动性AOSD患者的平均sCD 163（8.6 +/- A 5.4 mg/L）高于“非活动性”患者（4.6 +/- A 2.7 mg/L，p = 0.02）。AOSD（6.9 +/- A 4.9 mg/L）和脓毒症（7.1 +/- A 5.6 mg/L）的平均sCD 163高于HC（2.56 +/- A 1.17 mg/L，p < 0.001），但AOSD和脓毒症之间没有检测到差异。sCD 163与铁蛋白仅在AOSD中呈正相关（p = 0.0045; r = 0.4755）。活动性AOSD患者的血清铁蛋白（平均3，640.1 +/-A6，896.9 A μ g/L）高于脓毒症患者（1，720.2 +/-A3，882.1 A μ g/L，p < 0.007）。CD 163在淋巴结和扁桃体的B区和T区均匀分布。与扁桃体无关，铁蛋白仅在淋巴结B区表达。sCD 163是AOSD疾病活动性的标志物。与铁蛋白的相关性可能导致假设与高铁蛋白血症相关的巨噬细胞活化。铁蛋白仅在AOSD淋巴结的B区表达，提示该分子在疾病发病机制中作为抗原的作用。
Ferritin has a key role in Adult-onset Still's disease (AOSD). Its production seems related to macrophage activation of which sCD163 is a major serum marker. Thus, we aimed at evaluating the role of sCD163 in AOSD and its relationship with ferritin. Furthermore, we determined the expression of CD163 and ferritin in a lymph-node from an AOSD patient. sCD163 and serum ferritin were measured in 34 patients with AOSD (21 active, 13 non-active), 18 sepsis and 22 healthy controls (HC). Immunohistology was performed on a lymph-node from an AOSD patient in order to detect CD163 and ferritin. A tonsil from an HC was used as control. Mean sCD163 (8.6 +/- A 5.4 mg/L) was higher in active AOSD than "non-active" patients (4.6 +/- A 2.7 mg/L, p = 0.02). The mean sCD163 in AOSD (6.9 +/- A 4.9 mg/L) and sepsis (7.1 +/- A 5.6 mg/L) were higher than in HC (2.56 +/- A 1.17 mg/L, p < 0.001), but no difference between AOSD and sepsis was detected. sCD163 positively correlated with ferritin (p = 0.0045; r = 0.4755) only in AOSD. Serum ferritin (mean 3,640.1 +/- A 6,896.9 A mu g/L) was higher in active AOSD than in sepsis (1,720.2 +/- A 3,882.1 A mu g/L, p < 0.007). CD163 was equally distributed in the B and T areas of both lymph-node and tonsil. Differently from the tonsil, ferritin was expressed only in the lymph-node B area. sCD163 is a marker of disease activity in AOSD. The correlation with ferritin may lead to hypothesize a macrophage activation related to hyperferritinemia. Ferritin was found expressed only in the B area of the AOSD lymph-node, suggesting a role for this molecule as an antigen in the disease pathogenesis.