Immigrating progenitor cells contribute to human podocyte turnover
Immigrating progenitor cells contribute to human podocyte turnover
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DOI：
10.1038/sj.ki.5002524
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发表时间：
2007-12-01
影响因子：
19.6
通讯作者：
Hoyer, P. F.
中科院分区：
文献类型：
--
作者：
Becker, J. U.;Hoerning, A.;Hoyer, P. F.
足细胞耗竭是肾小球疾病中的一个关键事件，尤其是局灶节段性肾小球硬化症的发展。祖细胞迁移是一种可能的机制，足细胞替代肾单位功能的保护，因为，除了罕见的例外，成熟的足细胞被认为是无法复制。我们检查了8个石蜡包埋的肾活检，从6个男性受体的女性移植肾的受体来源的足细胞。Y染色体的荧光原位杂交与足细胞标记物Wilms肿瘤-1抗原的免疫荧光相结合。受体衍生的足细胞被发现在4/8活检代表3/6例患者。总体而言，在女性捐赠的肾脏中检查的740个足细胞中有5个是男性来源的。我们的研究表明，移民祖细胞能够取代足细胞在人类，然而，这一过程中的重要性，在生理和病理条件是未知的。
Podocyte depletion is a critical event in glomerular diseases in general and in the development of focal segmental glomerulosclerosis in particular. Progenitor cell immigration is a possible mechanism of podocyte replacement for the preservation of nephron function since, with rare exception, mature podocytes are thought to be incapable of replication. We examined eight paraffin-embedded renal biopsies from six male recipients of female transplant kidneys for receiver-derived podocytes. Fluorescent in situ hybridization for the Y chromosome was combined with immunofluorescence for the podocyte marker, Wilms tumor-1 antigen. Recipient-derived podocytes were found in 4 of 8 biopsies representing 3 of the 6 patients. Overall, 5 of the 740 podocytes examined in the female-donated kidneys were male derived. Our study suggests that immigrating progenitor cells are able to replace podocytes in humans; however, the importance of this process in physiologic and pathologic conditions is unknown.