Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
复制标题
用于多重检测的超亮荧光二氧化硅纳米颗粒
DOI：
10.3390/nano10050905
复制
发表时间：
2020-05-01
期刊：
影响因子：
5.3
通讯作者：
Sokolov, Igor
中科院分区：
文献类型：
--
作者：
Peerzade, Saquib Ahmed M. A.;Makarova, Nadezda;Sokolov, Igor
荧光标记是生物医学研究中的一种常用方法。同时使用具有不同但可分辨的荧光光谱的多个标记物（多路复用），例如在流式细胞术方法中，可以获得关于各种生化反应和疾病的更全面和更快的信息。在这里，我们报告的第一个演示的合成超亮荧光二氧化硅纳米多孔纳米粒子（星点），它有大量的复杂的荧光光谱适合多路复用的应用。光谱通过在合成浴中简单物理混合不同的市售荧光染料获得。所得的颗粒含有包封在二氧化硅基质的圆柱形纳米通道内的染料分子。染料分子之间的距离足够小，以在一部分包封的染料分子内实现福斯特共振能量转移（FRET）偶联。因此，可以有多个光谱的粒子，可以只用一个波长激发。我们展示了五种、三种和两种染料的混合情况。此外，染料可以以不同的比例混合在颗粒内部。这在所获得的光谱的复杂性中带来了另一个维度，并且使得不同的可分辨光谱的数量实际上是无限的。我们证明，通过不同的混合，只是两种染料内的每个颗粒得到的光谱可以很容易地区分使用线性分解方法。作为一个实际的例子，解复用的错误进行测量时，一百个粒子的组被用于标记。
Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix. The distance between the dye molecules is sufficiently small to attain Forster resonance energy transfer (FRET) coupling within a portion of the encapsulated dye molecules. As a result, one can have particles of multiple spectra that can be excited with just one wavelength. We show this for the mixing of five, three, and two dyes. Furthermore, the dyes can be mixed inside of particles in different proportions. This brings another dimension in the complexity of the obtained spectra and makes the number of different resolvable spectra practically unlimited. We demonstrate that the spectra obtained by different mixing of just two dyes inside of each particle can be easily distinguished by using a linear decomposition method. As a practical example, the errors of demultiplexing are measured when sets of a hundred particles are used for tagging.