Activated T lymphocytes produce a matrix-degrading heparan sulphate endoglycosidase
Activated T lymphocytes produce a matrix-degrading heparan sulphate endoglycosidase
复制标题
活化的 T 淋巴细胞产生基质降解硫酸乙酰肝素糖苷内切酶
作者：
Y. Naparstek;I. Cohen;Z. Fuks;I. Vlodavsky
我们先前发现，对髓鞘碱性蛋白(BP)具有自身敏感性的活化T淋巴细胞系，在静脉接种到同基因大鼠后，能够穿透血管，在神经系统内积聚，并导致实验性自身免疫性脑脊髓炎(EAE)1。一个重要的问题是，效应性T细胞如何到达血管壁外的这些靶点。为此，我们在体外研究了抗BP效应T淋巴细胞与血管内皮细胞产生的基底膜样细胞外基质的相互作用。我们现在报道，激活的但不是静止的T淋巴细胞产生一种内切糖苷酶，能够降解细胞外基质的蛋白多糖支架的硫酸乙酰肝素侧链。此外，抗BP的T淋巴细胞对细胞外基质递呈的BP的反应是通过显著增强内切糖苷酶的表达来实现的。这些结果表明，血管壁上的组织特异性抗原可以通过激活促进内皮下基底膜穿透的酶来引导淋巴细胞归巢。他们还表明，效应性T淋巴细胞可以识别与主要组织相容性复合体信号无关的抗原。
We have previously found that lines of activated T lymphocytes specifically autosensitized to the basic protein of myelin (BP), on intravenous inoculation into syngeneic rats, were able to penetrate blood vessels, accumulate in the nervous system and cause experimental autoimmune encephalomyelitis(EAE)1. An important question is how effector T cells reach such targets outside the walls of blood vessels. To investigate this we have studied in vitro the interaction of anti-BP effector T lymphocytes with the basement membrane-like extracellular matrix produced by vascular endothelial cells. We now report that activated but not resting T lymphocytes produce an endoglycosidase capable of degrading heparan sulphate side chains of the proteoglycan scaffold of the extracellular matrix. Moreover, the anti-BP T lymphocytes respond to BP presented by extracellular matrix by markedly enhanced elaboration of the endoglycosidase. These results suggest that tissue-specific antigens on blood vessel walls could direct lymphocyte homing by activating enzymes that facilitate penetration of the subendothelial basal lamina. They also suggest that effector T lymphocytes can recognize antigen which is not associated with a major histocompatibility complex signal.
影响因子：
3.4
作者：
Pu，Huangsheng;Gao，Peng;Lu，Hongbing
通讯作者：
Lu，Hongbing