Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine.
Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine.
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DOI：
10.1016/j.jmb.2009.02.066
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发表时间：
2009-05-01
影响因子：
5.6
通讯作者：
Jogl, Gerwald
中科院分区：
文献类型：
--
作者：
Demirci, Hasan;Belardinelli, Riccardo;Seri, Emilia;Gregory, Steven T.;Gualerzi, Claudio;Dahlberg, Albert E.;Jogl, Gerwald
Post-transcriptional modification of ribosomal RNA occurs in all kingdoms of life. The S-adenosyl-L-me-thionine-dependent methyltransferase KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Loss of this dimethylation confers resistance to the antibiotic kasugamycin. Here, we report biochemical studies and high-resolution crystal structures of KsgA from Thermus thermophilus. Methylation of 30S ribosomal subunits by T. thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate. The overall structure is similar to other methyltransferases but contains an additional α-helix in a novel N-terminal extension. Comparison of the apo-enzyme with complex structures with 5’-methylthioadenosine or adenosine bound in the cofactor-binding site reveal novel features when compared to related enzymes. Several mobile loop regions are observed that restrict access to the cofactor-binding site. In addition, the orientation of residues in the substrate-binding site indicates that conformational changes are required for binding two adjacent residues of the substrate rRNA.
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DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
14.9
作者：
HEUS, HA;VANKIMMENADE, JMA;HINZ, HJ
通讯作者：
HINZ, HJ
DOI：
10.1038/newbio233012a0
发表时间：
1971-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
HELSER, TL;DAVIES, JE;DAHLBERG, JE
通讯作者：
DAHLBERG, JE
影响因子：
4.8
作者：
Kurata, Shinya;Weixlbaumer, Albert;Suzuki, Tsutomu
通讯作者：
Suzuki, Tsutomu
DOI：
10.1038/84104
发表时间：
2001-02-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Goedecke, K;Pignot, M;Weinhold, E
通讯作者：
Weinhold, E