DETECTION OF STEROL HYDROPEROXIDES ON THIN-LAYER CHROMATOPLATES BY MEANS OF WURSTER DYES
DETECTION OF STEROL HYDROPEROXIDES ON THIN-LAYER CHROMATOPLATES BY MEANS OF WURSTER DYES
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DOI：
10.1016/s0021-9673(01)82933-2
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发表时间：
1972-01-01
期刊：
影响因子：
--
通讯作者：
HILL, FL
中科院分区：
文献类型：
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作者：
SMITH, LL;HILL, FL
开发了一种使用 N,N-二甲基对苯二胺二盐酸盐或 N,N,N',N'-四甲基对苯二胺二盐酸盐在冲洗薄层色谱上检测甾醇氢过氧化物的特异且灵敏的方法。使用颜色测试来确定胆固醇、豆甾醇、β-谷甾醇、羊毛甾醇、24,25-二氢羊毛甾醇、3α、5-环-5α-胆甾醇-6β-ol、5α-cholest-7-en-3β-ol 和 5α-cholest-8(14)-en-3β-ol 的老化样品中是否存在特定甾醇氢过氧化物，以及不存在 证明了类似储存的 5α-胆甾烷-3β-ol 和 5β-胆甾烷-3β-ol 样品中存在甾醇氢过氧化物。固态、辐照固态、胶体分散体和充气溶液中的胆固醇的自动氧化是通过最初形成甾醇氢过氧化物来进行的。
A specific and sensitive means of detection of sterol hydroperoxides on irrigated thin-layer chromatoplates using N,N-dimethyl-p-phenylenediamine dihydrochloride or N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride has been developed. Using the color test the presence of specific sterol hydroperoxides in aged samples of cholesterol, stigmasterol, β-sitosterol, lanosterol, 24,25-dihydrolanosterol, 3α, 5-cyclo-5α-cholestan-6β-ol, 5α-cholest-7-en-3β-ol, and 5α-cholest-8(14)-en-3β-ol and the absence of sterol hydroperoxides in similarly stored samples of 5α-cholestan-3β-ol and 5β-cholestan-3β-ol are demonstrated. Autoxidation of cholesterol in the solid state, in the irradiated solid state, in colloidal dispersion, and in aerated solution is shown to proceed via initial formation of sterol hydroperoxides.