Assay of urease activity in soils
Assay of urease activity in soils
复制标题
DOI：
10.1016/0038-0717(72)90064-8
复制
发表时间：
1972-11
影响因子：
9.7
通讯作者：
M. Tabatabai;J. M. Bremner
中科院分区：
文献类型：
--
作者：
M. Tabatabai;J. M. Bremner
本文介绍了一种测定土壤中尿素酶活性的简便、准确的方法。它包括测定土壤与三羟甲基氨基甲烷(THAM)缓冲液、尿素溶液和甲苯在37℃下孵育2小时时尿素酶活性释放的铵，通过用含有尿素酶抑制剂(Ag2SO4)的2.5M氯化钾处理培养的土壤样品，并用水蒸气蒸馏产生的土壤悬浮液3.3min来测定铵的释放。研究表明，用THAM缓冲液测定土壤尿素酶活力的最佳缓冲液pH为9.0，底物(尿素)浓度为0.02M，该方法适用于固铵土壤中尿素酶活力的测定。
A simple and precise method of assaying urease activity in soils is described. It involves determination of the ammonium released by urease activity when soil is incubated with tris(hydroxymethyl)aminomethane (THAM) buffer, urea solution, and toluene at 37°C for 2 h, ammonium release being determined by a rapid procedure involving treatment of the incubated soil sample with 2.5 M KC1 containing a urease inhibitor (Ag2SO4) and steam distillation of an aliquot of the resulting soil suspension with MgO for 3.3 min. Studies reported showed that the optimal buffer pH and substrate (urea) concentration for assay of soil urease activity using THAM buffer are 9.0 and 0.02 M, respectively, and that the method described is satisfactory for assay of urease activity in ammonium-fixing soils.