The use of molecular markers for pyramidizing resistance genes in grapevine breeding.
The use of molecular markers for pyramidizing resistance genes in grapevine breeding.
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在葡萄育种中使用分子标记金字塔化抗性基因。
DOI：
10.17660/actahortic.2009.827.96
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发表时间：
2009
影响因子：
2.2
通讯作者：
R. Töpfer
中科院分区：
文献类型：
--
作者：
R. Eibach;E. Zyprian;R. Töpfer
摘要 在 VHR 3082-1-42 x ‘Regent’ 杂交的 F1 后代中研究了使用分子标记的金字塔抗性基因的实际应用。 VHR 3082-1-42 是圆叶麝香 (Muscadinia rotundifolia) x 葡萄 (Vitis vinifera) 的杂交品种，与葡萄 (V. vinifera) 回交了四次（PAUQUET 等人，2001 年）。它携带抗白粉病的Run1基因和抗霜霉病的Rpv1基因。这两个基因均从圆叶麝香中引入（BOUQUET 等人，2000 年；WIEDEMANN-MERDINOGLU 等人，2006 年）。 “Regent”是一个对霜霉病和白粉病具有定量抗性的新品种（EIBACH 和 TOPFER 2003），于 1996 年在德国发布用于商业用途。使用 Run1 基因的分子标记（DONALD 等人，2002 年）、Rpv1 基因的两个 SSR 标记物（WIEDEMANN-MERDINOGLU 等人，2006 年）以及“Regent”的几个标记物对 F1 后代的 119 个个体进行了筛选，这些标记物与白粉病和霜霉病抗性表现出良好的相关性（ZYPRIAN 等人，2002 年，SALAKHUTDINOV 等人）等人 2003 年，AKKURT 等人 2004 年，FISCHER 等人 2004 年，AKKURT 等人 2007 年。通过叶盘的人工接种来进行霜霉病抗性的表型评估，通过温室中的自然感染来进行白粉病的表型评估。表型数据与分子标记分析结果的比较表明，抗性程度与抗性相关等位基因的存在之间存在明显的相关性。根据表型数据，20个基因型的子代均未感染白粉病和霜霉病。根据标记辅助评估，在这 20 种基因型中，有 4 种基因型携带所有与白粉病和霜霉病抗性相关的等位基因，表明来自双亲的抗性基因被有效组合。
Summary The practical application of pyramiding resistance genes by the use of molecular markers was investigated in a F1 progeny derived from the cross of VHR 3082-1-42 x ‘Regent’. VHR 3082-1-42 is a cross between Muscadinia rotundifolia x Vitis vinifera, backcrossed another four times with V. vinifera (PAUQUET et al. 2001). It carries the Run1-gene which causes resistance to powdery mildew and the Rpv1-gene which is related to resistance against downy mildew. Both genes were introduced from Muscadinia rotundifolia (BOUQUET et al. 2000; WIEDEMANN-MERDINOGLU et al. 2006). ‘Regent’ is a new cultivar with quantitative resistance against downy and powdery mildew (EIBACH and TOPFER 2003) released in Germany in 1996 for commercial use. 119 individuals of the F1 progeny were screened with a molecular marker for the Run1-gene (DONALD et al. 2002), with two SSR-markers for the Rpv1-gene (WIEDEMANN-MERDINOGLU et al. 2006) and with several markers from ‘Regent’ that showed good correlation to powdery and downy mildew resistance (ZYPRIAN et al. 2002, SALAKHUTDINOV et al. 2003, AKKURT 2004, FISCHER et al. 2004, AKKURT et al. 2007). Phenotypic evaluation for downy mildew resistance was done by artificial inoculation of leaf discs, and for powdery mildew by natural infection in a greenhouse. Comparison of the phenotypic data with the results of the molecular marker analyses showed a clear correlation between the degree of resistance and the presence of the resistance related alleles. According to the phenotypic data, 20 genotypes of the offspring were free of powdery and downy mildew infections. Based on a marker-assisted evaluation, out of these 20 genotypes a subset of four carried all the resistance related alleles for powdery and downy mildew indicating that resistance genes from both parents were effectively combined.