A micromethod for free erythrocyte porphyrins: the FEP test.

A micromethod for free erythrocyte porphyrins: the FEP test.
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游离红细胞卟啉的微量方法:FEP 测试。

DOI:
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发表时间:
1973
期刊:
Journal of Laboratory and Clinical Medicine
影响因子:
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通讯作者:
S. Piomelli
S. Piomelli
中科院分区:
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文献类型:
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作者:
S. Piomelli

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摘要 描述了一种简单快速的微量方法,用于测量 20 μl 血液中的游离红细胞卟啉(FEP 测试)。该技术利用简化的快速提取与荧光计的更高灵敏度相结合。用这种微方法获得的结果与传统技术的结果相比毫不逊色。当血铅水平增加时,游离红细胞卟啉浓度呈指数增加。微法(FEP 测试)似乎是筛查儿童铅中毒的一种很有前途的工具。
Abstract A simple and rapid micromethod for the measurement of free erythrocyte porphyrins (FEP test) from 20 μl of blood is described. The technique utilizes a simplified rapid extraction in combination with the greater sensitivity of the fluorometer. The results obtained with this micromethod compare favorably with those of a classical technique. The free erythrocyte porphyrin concentration increases exponentially when the blood lead level is increased. The micromethod (FEP test) appears to be a promising tool to screen children for lead poisoning.