In vivo imaging of human retinal flow dynamics by color Doppler optical coherence tomography.
In vivo imaging of human retinal flow dynamics by color Doppler optical coherence tomography.
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DOI：
10.1001/archopht.121.2.235
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发表时间：
2003-02
影响因子：
--
通讯作者：
S. Yazdanfar;A. Rollins;J. Izatt
中科院分区：
文献类型：
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作者：
S. Yazdanfar;A. Rollins;J. Izatt
背景技术彩色多普勒光学相干断层扫描(CDOCT)结合了激光多普勒测速和光学相干断层扫描，用于组织微结构和血流的同步微米级分辨率横截面成像。最近，CDOCT 被应用于裂隙灯生物显微镜，用于对人类视网膜的结构和血流进行成像。目的 证明 CDOCT 用于视网膜血流动力学成像的可行性。设计使 CDOCT 能够测量人类视网膜血流脉动。设置实验室。主要观察指标 时间分辨血流剖面和视网膜血流动力学图像，用于测量视网膜血管内的搏动。结果 在视神经乳头附近的选定血管上快速获取图像序列。从这些图像中提取视网膜血流轮廓并与光电体积描记器获得的外部参考同步。每个配置文件的获取时间不到 10 毫秒。结论 我们的结果表明，除了传统的光学相干断层扫描之外，CDOCT 还提供激光多普勒信息，允许在成像视网膜结构的同时观察血流动力学。 CDOCT 是一项用于视网膜血流动力学研究和临床研究的有前途的技术。临床相关性 血流动力学（例如搏动性和自动调节）已被证明在糖尿病视网膜病变和青光眼的整个进展过程中发生变化。使 CDOCT 能够观察视网膜动态，提高了其作为临床诊断工具的潜力。
BACKGROUND Color Doppler optical coherence tomography (CDOCT) combines laser Doppler velocimetry and optical coherence tomography for simultaneous micron-scale resolution cross-sectional imaging of tissue microstructure and blood flow. Recently, CDOCT was adapted to a slitlamp biomicroscope for imaging structure and blood flow in the human retina. OBJECTIVE To demonstrate feasibility of CDOCT for imaging retinal hemodynamics. DESIGN Enabling CDOCT to measure retinal blood flow pulsatility in humans. SETTING Laboratory. MAIN OUTCOME MEASURES Time-resolved flow profiles and images of retinal blood flow dynamics for measurement of pulsatility within retinal vessels. RESULTS Rapid sequences of images were acquired over selected vessels near the optic nerve head. From these images, retinal blood flow profiles were extracted and synchronized to an external reference obtained with a photoplethysmograph. Each profile was acquired in less than 10 milliseconds. CONCLUSIONS Our results indicate that CDOCT provides laser Doppler information in addition to conventional optical coherence tomography, allowing the observation of blood flow dynamics simultaneous to imaging retinal structure. CDOCT is a promising technology for research and clinical studies of retinal blood flow dynamics. CLINICAL RELEVANCE Blood flow dynamics, such as pulsatility and autoregulation, have been shown to change throughout the progression of diabetic retinopathy and glaucoma. Enabling CDOCT to observe retinal dynamics improves its potential as a clinical diagnostic tool.