Effects of epidermal growth factor, insulin-like growth factor-I, and dialyzed porcine follicular fluid on porcine oocyte maturation in vitro.
Effects of epidermal growth factor, insulin-like growth factor-I, and dialyzed porcine follicular fluid on porcine oocyte maturation in vitro.
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表皮生长因子、胰岛素样生长因子-I 和透析猪卵泡液对猪卵母细胞体外成熟的影响。
DOI：
10.1002/jez.1402660111
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Petters,RM
中科院分区：
文献类型：
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作者：
Reed,ML;Estrada,JL;Illera,MJ;Petters,RM
猪卵母细胞体外成熟过程中需要可能影响核成熟和/或细胞质成熟的未定义的卵泡因子。评估了表皮生长因子（EGF）、胰岛素样生长因子-I（IGF-I）和透析猪卵泡液（dpFF）对体外猪卵母细胞核成熟的影响。在实验 I 中，采用裂区因子设计制作了八种不同的成熟培养基，其中 dpFF（0% 与 10% v/v 透析 pFF）作为整个图成分，EGF（0.0 与 50 ng/ml）和/或 IGF-I（0.0 与 100 ng/ml）作为因子子图成分。实验 II 是使用 dpFF 和 EGF 的完整因子设计。猪卵泡颗粒-卵丘-卵母细胞复合物 (GCOC) 取自屠宰场卵巢，清洗并在 38.5°C、空气中含有 5% CO2 的加湿培养箱中培养 42 小时。培养后，GCOC 被机械剥离颗粒丘细胞，并通过光学显微镜评估核成熟情况。在实验 I 中，对照组、IGF-I、EGF 和 IGF-I + EGF 处理（无 pFF）的中期 II 卵母细胞百分比分别为 50.7%、52.6%、80.9% 和 84.3%（对照组和 IGF-I 组显着较低，P<.001）。存在 pFF 的相同处理相似且较高（分别为 84.2、84.9、82.1 和 86.8%）。实验二给出了类似的结果。这些结果表明，在不存在 pFF 的情况下，EGF 促进卵母细胞核成熟的水平与单独的 pFF 或 pFF 与 EGF 和/或 IGF-I 的促进水平相似。 IGF-I 似乎不影响 GCOC 的核成熟。 © 1993 Wiley-Liss, Inc.
Undefined follicular factors that may influence nuclear maturation and/or cytoplasmic maturation are required during in vitro maturation of pig oocytes. Epidermal growth factor (EGF), insulin‐like growth factor‐I (IGF‐I), and dialysed porcine follicular fluid (dpFF) were evaluated for their effects on porcine oocyte nuclear maturation in vitro. In Experiment I, eight different maturation media were made in a split‐plot factorial design with dpFF (0% vs. 10% v/v dialyzed pFF) as the whole plot component, and EGF (0.0 vs. 50 ng/ml) and/or IGF‐I (0.0 vs. 100 ng/ml) as the factorial subplot component. Experiment II was a complete factorial design with dpFF and EGF. Pig follicular granulosa‐cumulus‐oocyte complexes (GCOC) were obtained from slaughterhouse ovaries, washed, and cultured at 38.5°C in a humidified incubator with 5% CO2in air for 42 h. Following culture, GCOC were mechanically stripped of granulosa‐cumulus cells and evaluated for nuclear maturation by light microscopy. In Experiment I, the percentage of Metaphase II oocytes for control, IGF‐I, EGF, and IGF‐I + EGF treatments without pFF were 50.7%, 52.6%, 80.9%, and 84.3% (control and IGF‐I groups significantly less,P<.001). The same treatments in the presence of pFF were similar and high (84.2, 84.9, 82.1, and 86.8%, respectively). Experiment II gave similar results. These results demonstrate that EGF, in the absence of pFF, promotes a similar level of oocyte nuclear maturation as does pFF alone or pFF with EGF and/or IGF‐I. IGF‐I does not appear to influence nuclear maturation of GCOC. © 1993 Wiley‐Liss, Inc.