Generation of the Brucella melitensis ORFeome version 1.1
Generation of the Brucella melitensis ORFeome version 1.1
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DOI：
10.1101/gr.2456204
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发表时间：
2004-10-01
期刊：
影响因子：
7
通讯作者：
De Bolle, X
中科院分区：
文献类型：
--
作者：
Dricot, A;Rual, JF;De Bolle, X
布鲁氏菌属的细菌是一种世界性的人畜共患病，称为布鲁氏菌病。它们属于α-变形菌群，就像许多其他与真核宿主密切相关的细菌一样。重要的是，布鲁氏菌主要是细胞内病原体，其毒力的分子机制仍然知之甚少。利用羊种布氏杆菌全基因组序列，我们建立了一个蛋白质编码开放阅读框架（ORF）数据库，并构建了一个包含391个Gateway Entry克隆的ORF组文库，每个克隆包含3个定义的ORF。第一个版本的布鲁氏菌ORFeome（v1.1）以用户友好的格式提供编码序列，适合高通量功能基因组和蛋白质组学实验，因为ORF可以通过重组克隆方便地从入门克隆转移到各种表达载体。布鲁氏菌ORFeome v1.1的克隆将有助于更好地了解毒力的分子机制，包括鉴定细菌蛋白质-蛋白质相互作用，以及细菌效应子与其宿主靶标之间的相互作用。
The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. Using the complete genome sequence of Brucella melitensis, we generated a database of protein-coding open reading frames (ORFs) and constructed an ORFeome library of 3()91 Gateway Entry clones, each containing,3 defined ORF. This first version of the Brucella ORFeome (v1.1) provides the coding sequences in a user-friendly format amenable to high-throughput functional genomic and proteomic experiments, as the ORFs are conveniently transferable from the Entry clones to various Expression vectors by recombinational cloning. The cloning of the Brucella ORFeome v1.1 should help to provide a better understanding of the molecular mechanisms of virulence, including the identification of bacterial protein-protein interactions, but also interactions between bacterial effectors and their host's targets.