Maternal Huluwa dictates the embryonic body axis through β-catenin in vertebrates
Maternal Huluwa dictates the embryonic body axis through β-catenin in vertebrates
复制标题
母体葫芦娃通过脊椎动物中的β-连环蛋白决定胚胎体轴
DOI：
10.1126/science.aat1045
复制
发表时间：
2018-11-23
期刊：
影响因子：
56.9
通讯作者：
Meng, Anming
中科院分区：
文献类型：
--
作者：
Yan, Lu;Chen, Jing;Meng, Anming
脊椎动物的身体是由胚胎发育过程中的细胞运动和形状变化形成的。它仍然是不确定的母亲的信号支配的背组织者和体轴的形成。我们发现，母亲耗尽huluwa，以前未命名的基因，导致背部组织者，头部和身体轴在斑马鱼和爪蟾胚胎的损失。Huluwa蛋白存在于斑马鱼早期囊胚的背侧区卵裂球的质膜上。葫芦娃具有强烈的背侧化和次级轴诱导活性，这需要β-连环蛋白，但可以独立于Wnt配体/受体信号传导发挥作用。在机制上，葫芦娃结合并促进端锚聚合酶介导的Axin降解。因此，母体葫芦娃是脊椎动物胚胎背侧组织器和体轴的重要决定因素。
The vertebrate body is formed by cell movements and shape change during embryogenesis. It remains undetermined which maternal signals govern the formation of the dorsal organizer and the body axis. We found that maternal depletion of huluwa, a previously unnamed gene, causes loss of the dorsal organizer, the head, and the body axis in zebrafish and Xenopus embryos. Huluwa protein is found on the plasma membrane of blastomeres in the future dorsal region in early zebrafish blastulas. Huluwa has strong dorsalizing and secondary axis-inducing activities, which require beta-catenin but can function independent of Wnt ligand/receptor signaling. Mechanistically, Huluwa binds to and promotes the tankyrase-mediated degradation of Axin. Therefore, maternal Huluwa is an essential determinant of the dorsal organizer and body axis in vertebrate embryos.