Urinary cytokines in Schistosoma haematobium-infected schoolchildren from Tana Delta District of Kenya.
Urinary cytokines in Schistosoma haematobium-infected schoolchildren from Tana Delta District of Kenya.
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DOI：
10.1186/1471-2334-14-501
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发表时间：
2014-09-15
影响因子：
3.7
通讯作者：
Estambale BB
中科院分区：
文献类型：
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作者：
Njaanake KH;Simonsen PE;Vennervald BJ;Mukoko DA;Reimert CM;Gachuhi K;Jaoko WG;Estambale BB
血吸虫感染引起的病理变化包括细胞因子介导的尿路炎症。所涉及的细胞因子可能在尿液中排泄，因此它们在尿液中的存在可能反映了血结核杆菌相关的尿路病理。本研究首次报道了来自血吸虫高度感染地区的儿童尿液中选定的细胞因子与血吸虫感染的关系。对来自肯尼亚塔纳三角洲地区两所小学的5-12岁儿童进行了尿液滤过技术检查血结核杆菌卵，用Dipstix检查血尿，用ELISA法检查嗜酸性粒细胞阳离子蛋白、IL-6、干扰素-γ、肿瘤坏死因子-α和IL-10水平，并用超声检查血吸虫相关尿路病理。采用双抗体夹心法检测静脉血中IL-6、干扰素-γ、肿瘤坏死因子-α和IL-10水平。尿液与血清IL-6、干扰素-γ、肿瘤坏死因子-α、IL-10水平无明显相关性。轻度和重度血吸虫感染患儿的血清细胞因子、尿肿瘤坏死因子-α和干扰素-γ的几何平均强度无显著差异。而重度血吸虫感染组尿IL-6水平显著高于轻度感染组(p <； 0.001)，尿IL-10水平显著低于轻度感染组(p = 0.002)。尿IL-6与尿ECP呈显著正相关(p <； 0.001)，与尿ECP呈显著负相关(p = 0.012)。血吸虫感染患儿尿IL-6与感染强度、尿路炎症呈正相关，与尿路炎症呈负相关。尿液IL-6和IL-10可能是一种有用的非侵入性工具，可以补充已有的研究儿童血结核相关尿路病理的工具。本文的在线版本(DOI：10.1186/1471233414501)包含补充材料，可供授权用户使用。
Pathological changes due to infection with Schistosoma haematobium include cytokine-mediated urinary tract inflammation. The involved cytokines may be excreted in urine and their presence in urine may therefore reflect S. haematobium-related urinary tract pathology. The present study, for the first time, reports on the relationship between selected cytokines in urine and infection with S. haematobium in children from an area highly affected by this parasite. Children aged 5–12 years from two primary schools in Tana Delta District of Kenya were examined for S. haematobium eggs using urine filtration technique, for haematuria using dipstix and for eosinophil cationic protein (ECP), IL-6, IFN- γ, TNF-α and IL-10 levels using ELISA, and for S. haematobium-related urinary tract pathology using ultrasonography. In addition, venous blood was examined for serum IL-6, IFN- γ, TNF-α and IL-10 levels using ELISA. There was no significant correlation between urinary and serum levels of IL-6, IFN- γ, TNF-α or IL-10. There was no significant difference in geometric mean intensity (GMI) in any of the serum cytokines, or in urinary TNF-α or IFN-γ, between children with light and heavy S. haematobium infections. However, children with heavy S. haematobium infections had significantly higher GMI of urinary IL-6 (p < 0.001) and lower GMI of urinary IL-10 (p = 0.002) than children with light infections. There was also a significant positive correlation between urinary IL-6 and urinary ECP (p < 0.001) and a significant negative correlation between urinary IL-10 and urinary ECP (p = 0.012). Urinary IL-6 was positively correlated to and IL-10 was negatively correlated to infection intensity and urinary tract inflammation in S. haematobium-infected children. Urinary IL-6 and IL-10 ELISA may be a useful non-invasive tool to complement the already available tools for studying S. haematobium-related urinary tract pathology in children. The online version of this article (doi:10.1186/1471-2334-14-501) contains supplementary material, which is available to authorized users.