Multifunctional envelope-type nano device for non-viral gene delivery: concept and application of Programmed Packaging.
Multifunctional envelope-type nano device for non-viral gene delivery: concept and application of Programmed Packaging.
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DOI：
10.1016/j.jconrel.2007.06.018
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发表时间：
2007-10
期刊：
影响因子：
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通讯作者：
K. Kogure;H. Akita;H. Harashima
中科院分区：
文献类型：
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作者：
K. Kogure;H. Akita;H. Harashima
在这篇综述中，提出了一种称为“程序化包装”的新概念，用于开发非病毒基因递送系统。基于这一概念，开发了一种多功能包膜型纳米器件(MEND)，它可以通过巨噬细胞吞噬进入细胞，并像腺病毒一样有效地促进转染活动。优化了MEND的胞内转运，以防止溶酶体降解和加强核转位。还建立了定量分析方法(CIDIQ)来评估基因和携带者在细胞内运输的每一步。脂联素和腺病毒的比较研究表明，包括转录过程、翻译过程等在内的核内处置可能是脂联素Plus的限制步骤。这些研究表明，在开发有效的非病毒基因递送系统时，控制核内处置的重要性。
In this review, a new concept called “Programmed Packaging” is proposed for developing non-viral gene delivery systems. A Multifunctional Envelope-type Nano Device (MEND) was developed based on this concept, which can enter into cells via macropinocytosis and facilitate transfection activities as efficiently as an adenovirus. Intracellular trafficking of MEND was optimized in order to prevent lysosomal degradation and to enhance nuclear translocation. A quantitative analytical method (CIDIQ) was also established to evaluate each step in intracellular trafficking of genes as well as carriers. A comparative study between Lipofectamin and an adenovirus showed that intra-nuclear disposition, which includes the transcriptional process, translational process, etc., can be the limiting step for Lipofectamin PLUS. These studies indicated the importance of controlling intra-nuclear disposition in the development of an efficient non-viral gene delivery system.