HUMAN-ERYTHROCYTES SHED EXOCYTIC VESICLES INVIVO
HUMAN-ERYTHROCYTES SHED EXOCYTIC VESICLES INVIVO
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DOI：
10.1046/j.1537-2995.1984.24685066807.x
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
GREENWALT, TJ
中科院分区：
文献类型：
--
作者：
DUMASWALA, UJ;GREENWALT, TJ
研究旨在证明红细胞（RBC）是否在体内失去膜节段。将从收集在柠檬酸-磷酸-腺苷-腺嘌呤-酮中的新鲜血液中分离的血浆通过0.8 μ L的缓冲液。硝酸纤维素过滤器以除去残留的RBC和有形成分。通过在70，000 × 10 - 4离心分离胞吐囊泡。G.透射电子显微镜观察可见直径为50-200 nm的囊泡，其中含有Hb。囊泡膜十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示血影蛋白带1和2以及锚蛋白带2.1缺失。这些观察结果初步证明，红细胞脱落胞吐囊泡在正常循环。
Studies were designed to demonstrate whether red cells (RBC) lose membrane segments in vivo. Plasma separated from fresh blood collected in citrate-phosphate-dextrose-adenine-one was passed through 0.8 .mu. nitrocellulose filters to remove residual RBC and formed elements. Exocytic vesicles were separated by centrifugation at 70,000 .times. g. Transmission electron microscopy showed vesicles 50-200 nm in diameter which contained Hb. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of vesicle membranes showed that spectrin bands 1 and 2 and ankyrin band 2.1 were absent. These observations are the initial demonstration that RBC shed exocytic vesicles in the normal circulation.