Differential Regulation of Peripheral Leukocyte Genes in Patients With Active Crohn's Disease and Crohn's Disease in Remission
Differential Regulation of Peripheral Leukocyte Genes in Patients With Active Crohn's Disease and Crohn's Disease in Remission
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DOI：
10.1097/mcg.0b013e3181a9ef53
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发表时间：
2010-02-01
影响因子：
2.9
通讯作者：
Liew, Choong-Chin
中科院分区：
文献类型：
--
作者：
Burakoff, Robert;Hande, Scott;Liew, Choong-Chin
目的：评估疾病严重程度是克罗恩病管理中常见的挑战。迫切需要用于监测疾病活动的非侵入性、准确的标记物。与活动性克罗恩病相关的特定基因表达模式和分子生物标志物可以作为这样的标志物，从而提供一种新的方法来监测疾病的活动性。背景：循环白细胞中的基因表达谱已经显示出在几种医疗条件下的前景，血液可以提供一种容易获得的替代组织，用于使用基因表达谱来评估克罗恩病的活动性。在这项研究中，我们比较了全基因组的转录谱循环中的白细胞在患者活动和静止的克罗恩病。结果：我们观察到复杂的血液基因表达模式的变化，在活动性克罗恩病：各种功能类别的基因之间的差异调节活动性和非活动性克罗恩病。我们特别确定了一些炎症分子过度表达或表达不足的活动性克罗恩病，并验证了这些基因的一个子集，通过实时逆转录-聚合酶链反应.Conclusions：外周血白细胞差异调节的基因代表潜在的新的生物标志物，用于评估克罗恩病的活动。
Goals: Assessment of disease severity is a frequent challenge in the management of Crohn's disease. Noninvasive, accurate markers for monitoring disease activity are urgently required. Specific gene expression patterns and molecular biomarkers associated with active Crohn's disease could serve as such markers, thereby providing a novel approach to disease activity monitoring.Background: Gene expression profiling in circulating leukocytes has shown promise in several medical conditions and blood may provide an easily accessible surrogate tissue for using gene expression profiling to assess activity of Crohn's disease.Study: In this study, we compared genome-wide transcription profiles of circulating leukocytes in patients with active and quiescent Crohn's disease.Results: We observed complex changes in blood gene expression patterns in active Crohn's disease: genes of various functional categories were differentially regulated between active and inactive Crohn's disease. We specifically identified a number of inflammatory molecules overexpressed or underexpressed in active Crohn's disease and validated a Subset of these genes by real-time reverse transcription-polymerase chain reaction.Conclusions: The genes differentially regulated in peripheral leukocytes represent potential new biomarkers for assessing the activity of Crohn's disease.