Amniotic fluid volume responses to amnio-infusion of amniotic fluid versus lactated Ringer's solution in fetal sheep.
Amniotic fluid volume responses to amnio-infusion of amniotic fluid versus lactated Ringer's solution in fetal sheep.
复制标题
胎羊羊水体积对羊水输注与乳酸林格氏溶液的反应。
DOI：
10.1016/j.jsgi.2004.02.006
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Cheung,CeciliaY
中科院分区：
文献类型：
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作者：
Brace,RobertA;Cheung,CeciliaY
目的：本研究验证了一种假设，即羊水（AF）羊膜内输注比乳酸林格氏液输注能产生更持续的AF容量增加。方法：对5只长期插管的妊娠晚期胎羊进行为期2天的研究，每天测量心房纤颤容积。在第1天的基线测量后，将1 L加热的、先前冷冻的AF或加热的乳酸林格氏液注入羊膜内60分钟。两天后，注入另一种液体。在第二周，以相反的顺序注入液体。采用方差分析（ANOVA）进行统计检验。结果：羊膜内输注1007±7 (SE) mL AF或林格液后（n = 20）， 1天后羊膜内潴留仅为中度（37.2%±7.9%,P < 0.001），与2天后无显著差异（16.5%±9.5%,P = 0.1）。心房颤动与乳酸林格氏液输注后心房颤动体积或输注顺序无显著差异。AF成分变化相似，除了pH值和碳酸氢盐浓度在乳酸林格液后立即降低，1天后恢复正常。乳酸林格氏液输注后乳酸房颤升高，2天后降至基线值。在输注后1天，胎儿尿流率增加了75%±24%，两组之间无差异。结论：羊水输注后2天内房颤体积的扩大似乎并不取决于房颤中通常存在的成分的微小差异或微量成分（如激素、细胞因子或生长因子）的存在。
Objective:The present study tested the hypothesis that an intra-amniotic infusion of amniotic fluid (AF) would produce a more sustained increase in AF volume than an infusion of lactated Ringer's solution.Methods:Five chronically catheterized, late-gestation fetal sheep were studied over two 5-day periods with AF volume measured daily. After baseline measurements on day 1, 1 L of either warmed, previously frozen AF or warmed lactated Ringer's solution was infused intra-amniotically over 60 minutes. Two days later, the other fluid was infused. During the second week, fluids were infused in the opposite order. Analysis of variance (ANOVA) was used for statistical testing.Results:Following intra-amniotic infusion (n = 20) of 1007 ± 7 (SE) mL of either AF or Ringer's solution, intra-amniotic retention of the infused fluid was only moderate after 1 day (37.2% ± 7.9%, P < .001) and was not significantly different from zero after 2 days (16.5% ± 9.5%, P = .1). There were no significant differences in AF volume following infusion of AF versus lactated Ringer's solution or the order in which they were infused. AF compositional changes were similar except that pH and bicarbonate concentration were reduced as expected immediately after lactated Ringer's solution with a return to normal values after 1 day. AF lactate increased after lactated Ringer's solution infusion, declining to baseline values after 2 days. Fetal urine flow rate increased by 75% ± 24% at 1 day postinfusion and there was no difference between infusates.Conclusions:The expansion of AF volume over 2 days following amnio-infusion does not appear to depend on minor compositional differences or the presence of microconstituents such as hormones, cytokines, or growth factors that are normally present in AF.