Micro-computed tomography for studies on Entobia: transparent substrate versus modern technology
Micro-computed tomography for studies on Entobia: transparent substrate versus modern technology
复制标题
用于 Entobia 研究的微型计算机断层扫描：透明基底与现代技术
DOI：
--
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
G. Shields
中科院分区：
文献类型：
--
作者：
C. Schönberg;G. Shields
内石器生物侵蚀很难分析和描述，它通常需要破坏样品材料。海绵糜烂（Entobia）可能是最难评价的一种，因为它同时具有宏观不均匀性和微观结构复杂性。我们研究了两种澳大利亚海绵Cliona celata和Cliona orientalis的生物侵蚀痕迹与现代技术：高分辨率X射线显微计算机断层扫描。Micro-CT可以在三维空间中对活的和死的结构进行非破坏性可视化，并与传统的显微镜方法进行了比较。Micro-CT和显微镜观察表明，C.纤维素侵蚀在中心更强烈，并在周边分支（21%对9%去除基质）。与此相反，C. orientalis产生了一个密集的，甚至网络，并导致整体上更强烈的侵蚀模式比C。CELATA（48中央vs. 42%边缘基质去除）。扩展先锋长丝通常没有发现在边缘的研究海绵侵蚀，但分支突然结束或渐缩点。显微CT获得的结果在质量上与解剖显微镜下透明光学晶石的观察结果相似。显微结构无法像预期的那样用显微CT分辨。尽管海绵疤痕和海绵碎片在最大放大率的显微CT图像上很容易识别，但它们缺乏SEM提供的细节。微型CT的其他缺点涉及高成本和目前有限的访问。尽管显微CT目前还不能取代传统的技术，如环氧树脂铸件通过SEM观察，我们获得了有价值的信息。特别是对于测量石内孔隙体积的可能性，我们认为micro-CT是一个非常有前途的工具，将继续优化。不同方法的结合将在内托比亚的研究中产生最好的结果
Endolithic bioerosion is difficult to analyse and to describe, and it usually requires damaging of the sample material. Sponge erosion (Entobia) may be one of the most difficult to evaluate, as it is simultaneously macroscopically inhomogeneous and microstructurally intricate. We studied the bioerosion traces of the two Australian sponges Cliona celataand Cliona orientaliswith modern technology: high resolution X-ray micro-computed tomography. Micro-CT allows non-destructive visualisation of live and dead structures in three dimensions and was compared to traditional microscopic methods. Micro-CT and microscopy showed that C. celatabioerosion was more intense in the centre and branched out in the periphery (21 vs. 9% substrate removed). In contrast, C. orientalisproduced a dense, even meshwork and caused an overall more intense erosion pattern than C. celata(48 central vs. 42% marginal substrate removed). Extended pioneering filaments were not usually found at the margins of the studied sponge erosion, but branches ended abruptly or tapered to points. Results obtained with micro-CT were similar in quality to observations from transparent optical spar under the dissecting microscope. Microstructures could not be resolved as well with micro-CT as anticipated. Even though sponge scars and sponge chips were easily recognisable on maximum magnification micro-CT images, they lacked the detail that is available from SEM. Other drawbacks of micro-CT involve high costs and presently limited access. Even though micro-CT cannot presently replace traditional techniques such as epoxy resin casts viewed by SEM, we obtained valuable information. Especially for the possibility to measure endolithic pore volumes, we regard micro-CT as a very promising tool that will continue to be optimised. A combination of different methods will produce the best results in the study of Entobia