ACTIVE-TRANSPORT OF IONS ACROSS FROG RETINAL-PIGMENT EPITHELIUM
ACTIVE-TRANSPORT OF IONS ACROSS FROG RETINAL-PIGMENT EPITHELIUM
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DOI：
10.1016/0014-4835(77)90090-2
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发表时间：
1977-01-01
影响因子：
3.4
通讯作者：
STEINBERG, RH
中科院分区：
文献类型：
--
作者：
MILLER, SS;STEINBERG, RH
在短路状态下测量了穿过离体青蛙[Rana catesbiana]视网膜色素上皮-脉络膜制备物的离子通量。脉络膜到视网膜（基底到顶端）方向有 22Na+ (0.47[.mu.Eq/cm2 h]) 净通量，被顶端哇巴因 10-4 M 抑制 50-60%。未观察到该净通量的季节性变化。视网膜至脉络膜 36Cl- 的净通量随季节变化，春季较高 (0.5-0.74[.mu.Eq/cm2 h])，夏季较低 (0.2-0.5[.mu.Eq/cm2 h])。净 22Na+ 加 36Cl- 通量并不能解释春季或夏季的所有短路电流。乙酰唑酰胺 10-3 M 和沐浴溶液中 10 倍的减少同样抑制了净 36Cl-通量。**图形**。浓度(27.5-2.75 mM)。 36Cl-通量不受哇巴因的影响。净 22Na+ 和 36Cl- 通量的抑制表现出类似的变化模式。在这两种情况下，净传输方向的单向通量略有减少或根本没有减少，而相反的通量则显着增加。脉络膜到视网膜方向的 45Ca2+ 净通量为 4-5[nM/cm2 h]，是顶端哇巴因 (10-4 M) 的两倍，并且不受 .**GRAPHIC** 10 倍减少的影响。 [细胞外。**图形**。浓度]或乙酰唑酰胺，10-3 M。
Ion fluxes across the isolated frog [Rana catesbiana] retinal pigment epithelium-choroid preparation were measured in the short circuited state. There was a net flux of 22Na+ (0.47[.mu.Eq/cm2 h]) in the choroid to retina (basal to apical) direction that was 50-60% inhibited by apical ouabain, 10-4 M. No seasonal variation in this net flux was observed. The net retina to choroid 36Cl- flux did vary from season to season, being high (0.5-0.74[.mu.Eq/cm2 h]) in the spring, and low (0.2-0.5[.mu.Eq/cm2 h]) in the summer. The net 22Na+ plus 36Cl- flux did not account for all the short circuit current in either the spring or summer. The net 36Cl- flux was equally inhibited by acetazolamide, 10-3 M, and by a 10-fold reduction in the bathing solution .**GRAPHIC**. concentation concentration (27.5-2.75 mM). The 36Cl- fluxes were not affected by ouabain. The inhibition of the net 22Na+ and 36Cl- fluxes exhibited a similar pattern of changes. In both cases the unidirectional flux, in the direction of net transport, was slightly or not at all reduced, and the oposite flux was significantly increased. There was a net flux of 45Ca2+ in the choroid to retina direction of 4-5[nM/cm2 h] that was double by apical ouabain (10-4 M) and not affected by a 10-fold reduction in .**GRAPHIC**. [extracellular .**GRAPHIC**. concentration] or acetazolamide, 10-3 M.