Hydrazino-s-triazine based labelling reagents for highly sensitive glycan analysis via liquid chromatography-electrospray mass spectrometry.
Hydrazino-s-triazine based labelling reagents for highly sensitive glycan analysis via liquid chromatography-electrospray mass spectrometry.
复制标题
DOI：
10.1016/j.talanta.2015.07.045
复制
发表时间：
2015-11
期刊：
影响因子：
6.1
通讯作者：
Ming-Zhe Zhao;Yiwei Zhang;Fang Yuan;Yan Deng;Jing-Xin Liu;Yinglin Zhou;Xinxiang Zhang
中科院分区：
文献类型：
--
作者：
Ming-Zhe Zhao;Yiwei Zhang;Fang Yuan;Yan Deng;Jing-Xin Liu;Yinglin Zhou;Xinxiang Zhang
由于葡聚糖的高亲水性和低电离效率，标记策略在基于MS的葡聚糖分析中起着重要作用。本工作在简便、可控的条件下合成了10种肼-S-三氮杂基标记试剂，用于高灵敏的高效液相色谱-电喷雾-质谱法分析多糖。这些新的标记试剂通过非还原反应连接到N-糖链上，在不同程度上增强了糖链对质谱学的响应。以麦芽七糖(DP7)为模型，10种标记试剂中有3种具有良好的线性关系和较低的检出限。此外，以最适宜的标记剂为例，对从鸡抗生物素和人血清糖蛋白中切割的N-连接糖链进行了高灵敏的分析，表明这些标记剂在糖组学研究的前沿领域具有广阔的应用前景。
Labelling strategy plays an important role in mass spectrometry (MS) based glycan analysis due to the high hydrophilicity and low ionization efficiency of glycans. Ten hydrazino-s-triazine based labelling reagents were synthesized under facile and controllable conditions for highly sensitive liquid chromatography–electrospray mass spectrometry glycan analysis in this work. Attached to N-glycans through non-reductive reactions, these new labelling reagents were evaluated in aspect of the differently enhanced glycan response to mass spectrometry. Three of the ten labelling reagents demonstrated to be reliable and remarkable for glycan analysis with satisfactory linearity and lowered limits of detection using maltoheptaose (DP7) as model. Furthermore, the most optimal labelling reagent was taken as an example for highly sensitive profiling of N-linked glycans both cleaved from chicken avidin and glycoproteins in human serum, indicating prospective availability for these labelling reagents in frontier of glycomics researches.