Neuroprotective effects of Lycium barbarum polysaccharides in lipopolysaccharide-induced BV2 microglial cells.
Neuroprotective effects of Lycium barbarum polysaccharides in lipopolysaccharide-induced BV2 microglial cells.
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DOI：
10.3892/mmr.2013.1442
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发表时间：
2013-06
影响因子：
3.4
通讯作者：
P. Teng;Yunhong Li;Wenjing Cheng;Liang Zhou;Ying Shen;Yin Wang
中科院分区：
文献类型：
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作者：
P. Teng;Yunhong Li;Wenjing Cheng;Liang Zhou;Ying Shen;Yin Wang
枸杞多糖具有多种生物活性。然而，其神经保护作用尚未完全阐明。本研究的目的是研究LBP对BV 2小胶质细胞产生脂多糖（LPS）诱导的促炎介质的抑制作用。培养BV 2小鼠小胶质细胞，并进行MTT测定以确定LBP是否对LPS刺激的BV 2细胞的凋亡具有影响。我们的数据表明，LPS诱导核因子-κB（NF-κB）及其上游蛋白caspase 3的活化，上调BV 2小胶质细胞中另外一种凋亡诱导因子热休克蛋白60（HSP 60）的表达，并增加培养液中TNF-α和HSP 60的释放。LBP处理后，活化的NF-κB和caspase 3被显著抑制。同时，还能抑制LPS诱导的TNF-α和HSP 60的释放，降低HSP 60的表达。这些结果表明，LBP可能具有治疗伴有小胶质细胞活化的神经退行性疾病的治疗潜力。
Polysaccharides extracted from Lycium barbarum (LBPs) possess a wide variety of biological activities. However, their neuroprotective effects have not yet been fully elucidated. The aim of the present study was to investigate the inhibitory effects of LBPs on the production of lipopolysaccharide (LPS)‑induced proinflammatory mediators in BV2 microglia. BV2 mouse microglial cells were cultured and an MTT assay was performed to determine whether LBPs had an effect on the apoptosis of LPS-stimulated BV2 cells. Our data showed that LPS induced the activation of nuclear factor‑κB (NF‑κB) and its upstream protein caspase 3, upregulated the expression of an additional apoptosis‑inducing factor, heat shock protein 60 (HSP60), in BV2 microglial cells and increased the release of TNF-α and HSP60 in the culture media. Following treatment with LBPs, the activated NF‑κB and caspase 3 were significantly suppressed. Furthermore, the enhanced expression of HSP60 was reduced and the LPS-induced release of TNF-α and HSP60 were inhibited. These results suggest that LBPs may have therapeutic potential for the treatment of neurodegenerative diseases that are accompanied by microglial activation.