Antibacterial Activity of Ag-Hydroxyapatite Coating Against Hematogenous Infection by Methicillin-Resistant Staphylococcus aureus in the Rat Femur.
Antibacterial Activity of Ag-Hydroxyapatite Coating Against Hematogenous Infection by Methicillin-Resistant Staphylococcus aureus in the Rat Femur.
复制标题
Ag-羟基磷灰石涂层对大鼠股骨耐甲氧西林金黄色葡萄球菌血行感染的抗菌活性。
DOI：
10.1002/jor.24431
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Mawatari M.
中科院分区：
文献类型：
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作者：
Kobatake T;Miyamoto H;Hashimoto A;Ueno M;Nakashima T;Murakami T;Noda I;Shobuike T;Sonohata M;Mawatari M.
已经开发出几种抗菌材料来预防假体周围关节感染，从而防止患者和外科医生出现严重并发症。然而，尚无研究探讨抗菌材料对抗血行感染的活性。本研究评估了含银羟基磷灰石涂层植入物对耐甲氧西林金黄色葡萄球菌（MRSA）血源性感染的抗菌活性。将涂有羟基磷灰石和银羟基磷灰石的植入物分别插入大鼠的右股骨和左股骨中，然后使动物感染链球菌。金黄色葡萄球菌通过尾部血管。约107个菌落形成单位是S菌建立的最佳细菌数。金黄色葡萄球菌血源性感染。测量血液中的细菌负荷和 C 反应蛋白以确认菌血症和炎症。感染后 14 天，含有银羟基磷灰石涂层植入物的股骨中的细菌载量在统计学上低于含有羟基磷灰石涂层植入物的股骨（p= 0.022）。因此，银羟基磷灰石涂层植入物可能在术后提供针对 MRSA 血行感染的抗菌活性。 © 2019 骨科研究会。 Wiley periodicals, Inc. 出版 J Orthop Res 37:2655–2660, 2019
Several antibacterial materials have been developed to prevent periprosthetic joint infection and thus prevent serious complications for patients and surgeons. However, no study has addressed the activity of antibacterial materials against hematogenous infection. The present study evaluated the antibacterial activity of a silver‐containing hydroxyapatite‐coated implant against methicillin‐resistantStaphylococcus aureus(MRSA) hematogenous infection. Implants coated with hydroxyapatite and silver‐hydroxyapatite were inserted into rats’ right and left femurs, respectively, after which the animals were infected withS. aureusvia a tail vessel. About 107colony‐forming units was the optimal bacterial number for the establishment ofS. aureushematogenous infection. Bacterial loads and C‐reactive protein in the blood were measured to confirm bacteremia and inflammation. Fourteen days after the infection, bacterial loads were statistically lower in the femurs containing silver‐hydroxyapatite‐coated implants than in those with hydroxyapatite‐coated implants (p= 0.022). Thus, silver‐hydroxyapatite‐coated implants might provide antibacterial activity against MRSA hematogenous infection in the postoperative period. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2655–2660, 2019