Lung level of HMBG1 is elevated in response to advanced glycation end product-enriched food in vivo
Lung level of HMBG1 is elevated in response to advanced glycation end product-enriched food in vivo
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DOI：
10.1002/mnfr.200600223
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发表时间：
2007-04-01
影响因子：
5.2
通讯作者：
Simm, Andreas
中科院分区：
文献类型：
--
作者：
Bartling, Babett;Fuchs, Christine;Simm, Andreas
高迁移率族蛋白1（HMGB1）是一种普遍存在的核蛋白，在某些条件下可以从细胞中主动释放，从而介导类精氨酸功能。虽然核HMGB1调节细胞的转录活性，但细胞外HMGB1部分通过结合晚期糖基化终产物（AGEs）受体发挥作用，AGEs在肺组织中高度表达。因此，我们研究了食物来源的晚期糖基化终产物（AGEs），美拉德反应产物，对HMGB1肺表达的影响。用富含AGE的饮食喂养大鼠，包括面包皮或咖啡饮料，导致HMGB1 mRNA和蛋白的上调，特别是在那些接受面包皮饮食的动物中。患者的表情无明显变化。此外，我们发现，在接受面包皮或咖啡提取物的两个动物组中，肺AGE水平增加与HMGB 1蛋白表达之间呈正相关，但在对照组中没有。与此相反，与衰老相关的AGE积累是不相关的HMGB1蛋白在肺组织中的水平增加，从衰老（100周）相比，成年（24周）大鼠。我们的数据表明，在调节HMGB1在肺中的表达的生理作用的食物，但不是年龄相关的AGEs。
High mobility group box protein 1 (HMGB1) is a ubiquitous nuclear protein that can be actively released from the cell in certain conditions thereby mediating cytokine-like function. While nuclear HMGB1 modulates the transcriptional activity of cells, extracellular HMGB1 partially acts via binding to the receptor for advanced glycation end products (RAGE), which is highly expressed in lung tissue. Therefore, we studied the impact of food-derived advanced glycation end products (AGEs), the Maillard reaction products, on the lung expression of HMGB1. Feeding rats with AGE-rich diet, containing either bread crust or coffee beverage, resulted in an upregulation of HMGB1 mRNA and protein especially in those animals receiving bread crust diet. The expression of RAGE was not influenced. Moreover, we revealed a positive correlation between an increased lung AGE level and HMGB 1 protein expression in both animal groups receiving either bread crust or coffee extract but not in the control group. In contrast, the ageing-related AGE accumulation was not associated with an increased level of HMGB1 protein in lung tissue from senescent (100 wk) compared to young-adult (24 wk) rats. Our data suggest a physiological role of food- but not ageing-associated AGEs in the regulation of the HMGB1 expression in lung.